Abstract: The present invention provides an in situ monitoring of the decomposing activity against oligonucleotides target in a biological tissue. A single-chain oligonucleotide target comprises an appropriate number of nucleic acid bases to be examined with an energy donor and an energy acceptor respectively at its 5'- and 3'-terminals. Monitoring of the fluorescence changes of the target after injection into a biological tissue, particularly the fluorescent resonance energy transfer(FRET) phenomena between the energy donor and acceptor, indicates whether the oligonucleotide is not decomposed yet.

Abstract: A process for production of an exogenous gene or its product in a plant cell which comprises: inserting into a genome of plant,i) cDNA of replicase gene from an RNA plant virus, andii) cDNA of a recombinant virus genomic RNA inwhich coat protein gene is wholly or partly replaced with desired exogenous gene, orinoculating said recombinant virus genomic RNA on a plant cell having cDNA of replicase gene inserted in the genome. The process of the present invention enables to efficiently produce the desired exogenous gene product in plant cells in large quantities.

Abstract: Method of desilylating a silylether compound by reacting a silylether compound of the general formula (I): ##STR1## or a salt thereof with an amine hydrogen fluoride salt or a pyridine hydrogen fluoride salt in an organic solvent to produce a compound of the general formula (IV): ##STR2## According to this method, silylether compounds that are labile under strong acidic or basic conditions can be desilylated efficiently using inexpensive reagents.

Abstract: The object of the present invention is to provide a reversible heat sensitive recording material capable of formation and erasion of images with a clear contrast and capable of retaining stable images with time under the conditions of daily living. A reversible heat sensitive recording material comprising a support and, provided thereon, a normally colorless or light colored dye precursor and a reversible color developer which causes reversible change of color tone in the dye precursor due to the difference in cooling rate after heating, characterized in that said reversible heat sensitive recording material contains at least one specific color erasion accelerator containing a long chain alkyl group.

Abstract: In a method for preventing dust of a powdery material by mixing the powdery material with a polytetrafluoroethylene and subjecting the mixture to a compression-shearing action taken at temperatures from about 20.degree. to 200.degree. C. thereby the polytetrafluoroethylene is fibrillated to prevent dusting of the powdery material, the method is characterized by that polytetrafluoroethylene is an aqueous emulsion of a homopolymer of tetrafluoroethylene, the homopolymer has a specific gravity of not larger than 2.20 and an average particle size of 0.10-0.50.mu., and the emulsion contains a hydrocarbon-base anionic surfactant of not less than 1.0 wt % of the polytetrafluoroethylene as the emulsion stabilizer. The emulsion is highly stable and a less possible contaminant of underground water.