Abstract: A method is provided for preconditioning and cryopreservation of cells harvested from a donor. Cells are suspended in a cell conditioning and cryopreservation medium containing a cryopreservative such as Dimethyl Sulfoxide (DMSO) and the suspension is incubated for a period of at least ten minutes and the cells are frozen. The medium includes adenosine, a calcium channel blocker, and a cell nutrient matrix comprising a sufficient amount of cell nutrients to sustain the metabolic needs of the cells during incubation without producing detectable levels of lactate or substantially depleting the nutrient substrates to maintain viability of the harvested cells. In some embodiments, a cryopreservative is added step-wise before the cell suspension is frozen and removed step-wise after the cell suspension is thawed.

Abstract: A machine tool center (10) displays at least one machine tool (11, 12, 13) which has a working area (17) accessible through an operator door (16) to process workpieces (21). Furthermore, a loading/unloading station (14) for the workpieces (21) and a loading device (22) which transports the workpieces (21) between the loading/unloading station (14) and the respective machine tool (11, 12, 13) and changes these at the machine tool (11, 12, 13) are also provided. In the new machine tool center (10) the loading device (22) is arranged below the working space (17) of the machine tool (11, 12, 13) (FIG. 1).

Abstract: A technique is described for identifying mutations, if any, present in a biological sample, from a pre-selected set of known mutations. The method can be applied to DNA, RNA and peptide nucleic acid (PNA) microarrays. The method analyzes a dot spectrogram representative of quantized hybridization activity of oligonucleotides in the sample to identify the mutations. In accordance with the method, a resonance pattern is generated which is representative of nonlinear resonances between a stimulus pattern associated with the set of known mutations and the dot spectrogram. The resonance pattern is interpreted to a yield a set of confirmed mutations by comparing resonances found therein with predetermined resonances expected for the selected set of mutations.

Abstract: A HHV-8 producing immortalized lymphoma cell line, which is free of EBV, CMV, and HIV, and which produces large quantities of uncontaminated HHV-8.

Abstract: The system, method, and program of this invention enables an object language application (e.g., C++, JAVA, etc.,) to issue a query over a view and to receive back, as query results, handles to application type objects which can be further manipulated by the application. A view is defined herein as a collection of a view type, and a view type is defined as a class or type. Upon receipt of the query referencing a view type, a query engine generates a query plan that builds mock (i.e., proxy) application type objects in memory based upon the view types. The application can run methods on the application type objects or point to other application type objects from the handles, to the application objects, that are returned to the application; and these manipulations will be understood by the query engine.

Abstract: A method, apparatus, and article of manufacture for a computer-implemented random reliability engine for computer-implemented association rule reduction using association rules for data mining application. The data mining is performed by the computer to retrieve data from a data store stored on a data storage device coupled to the computer. The data store has records that have multiple attributes. Attribute value associations are determined between attributes and their values. Attribute associations are determined from the determined attribute value associations. Attributes are selected based on the determined attribute associations for performing data mining.

Abstract: A system for dispensing liquids, particularly a system for dispensing liquid soap through at least one hand-operated pump. The system includes a pump, a main reservoir for holding a first quantity of the fluid to be dispensed, and an auxiliary reservoir for holding a second and generally smaller quantity of fluid. The main reservoir, auxiliary reservoir, and pump are in fluid communication with one another through a coupling. The system and coupling are configured so that the fluid is drawn preferentially from the main reservoir, with fluid being drawn from the auxiliary reservoir only when the main reservoir is substantially emptied of fluid. Additionally, any fluid that is drawn from the auxiliary reservoir will be replenished automatically by fluid in the main reservoir as long as any fluid is present in the main reservoir.

Abstract: A sealant for forming a fluid seal between the faying surface of two metallic substrates that are compressively and mechanically joined together. The sealant is a liquid in its pre-cured condition and a dry solid in its cured condition. The transition of the sealant from its pre-cured condition to its cured condition occurs at room temperature. The sealant's curing time is not less than about 10 minutes and not more than about 16 hours. The cured sealant has a Shore A hardness between about 30 and 70. The cured sealant is impermeable and resistant to chemical attack by air, water, common solvents and petroleum fuels. Also, the cured sealant is resistant to compressive cold flow, however, the cured sealant is deformable to conform to an abutting surface or with a layer of similar sealant. The cured sealant is flexible and resistant to temperatures between about -65 degrees F. and about 250 degrees F.

Abstract: A method of reducing the stress in meat producing animals and of improving the quality of the meat produced from slaughtering said animals, while reducing the number of animals lost due to incapacitation, injury, or untimely death, by administering to the animals an effective amount of epinephrine or an epinephrine-like drug sufficient to acclimate the animal, and allowing the animal then to rest before loading the animal to be slaughtered by industry standard procedures for such meat producing animals. This method produces a meat with high sensory qualities.

Abstract: A washing system for dispensing a liquid cleanser from a collapsible container, with a plurality of dispensers, each dispenser having a positive displacement pump, a mount for mounting the pump on a dispenser support, and a connection for connecting the pump to a flexible supply line, a container support for supporting a collapsible container of liquid cleanser, and a flexible supply line for connecting the collapsible container to each of the pumps, with a one-way valve in the line for blocking fluid flow to the collapsible container while permitting fluid flow from the collapsible container to the dispensers. A secondary reservoir may be provided to permit continued service when the main reservoir is empty or is being replaced.

Abstract: Disclosed is an increased strength dogging mechanism operable by a handle for releasably securing a door on each side of the door to a structure. The dogging mechanism includes a plurality of dog keepers each having a ramp portion and a seating portion mounted to each side of the door. A dog having a roller is rotatably mounted to engage a cooperatively associated dog keeper. A mounting bolt extends through the structure and through the approximate center of each dog. A drive assembly which is movable by the handle between open and closed positions moves each dog, simultaneously, between unlocked and locked positions. As the dog is rotated to the locked position, the roller of each dog engages the ramp portion of its associated dog keeper and rolls upon the ramp and into the seating portion.

Abstract: A spa jet comprising a first nozzle, a second nozzle, and an aeration chamber therebetween, are all disposed in a housing. One seal forms a watertight seal between the first nozzle and the housing, and another seal forms a watertight seal between the second nozzle and the housing. A ring is affixed to the second-nozzle downstream end, and a third nozzle is pivotally mounted in the ring. A retainer is mounted to the ring and it frictionally secures the third nozzle in any one of a continuum of angular positions. The ring can be a ball-bearing ring, whereby the third nozzle can freely rotate in the ball-bearing ring under the force of a water stream exiting the third nozzle. A non-circular opening in the third nozzle assists in rotating the third nozzle even when centered in the ring. A barrel, comprising the first and second nozzles and the aeration chamber, has latching tabs formed thereon, and the housing has a retaining profile formed therein for engaging the latching tabs.

Abstract: The Laser Induced Fluorescence Attenuation Spectroscopy (LIFAS) method and apparatus preferably include a source adapted to emit radiation that is directed at a sample volume in a sample to produce return light from the sample, such return light including modulated return light resulting from modulation by the sample, a first sensor, displaced by a first distance from the sample volume for monitoring the return light and generating a first signal indicative of the intensity of return light, a second sensor, displaced by a second distance from the sample volume, for monitoring the return light and generating a second signal indicative of the intensity of return light, and a processor associated with the first sensor and the second sensor and adapted to process the first and second signals so as to determine the modulation of the sample.

Abstract: A method, apparatus, and article of manufacture for a computer-implemented repartitioning system. Data is repartitioned in a database stored on a data storage device connected to a computer. First, it is detected that a partitioning scheme for the data has been altered. Next, partitions that would be affected by the altered partitioning scheme are identified. Then, the identified partitions are reorganized based on the altered partitioning scheme.