Abstract: A rolling circle DNA replicon which replicates in a host eukaryotic cell is disclosed which has a truncated replication cycle. The rolling circle DNA replicon comprises the following elements present on the same DNA molecule. It contains a Rep gene open reading frame from a virus belonging to the viral taxonomic families Geminiviridae, Circoviridae or genus Nanovirus. The Rep gene open reading frame is placed under transcriptional control of a promoter, which is placed 5? of the gene. Any sequences that are required to be present in cis on the rolling circle DNA replicon in order that the Rep protein might promote replication of the rolling circle DNA replicon are included. An expression cassette for expression of an ancillary protein that is capable of creating a cellular environment permissive for replication of the rolling circle DNA replicon in the host cell of interest is also included.

Abstract: This invention provides methods of identifying ligands that are internalized into a cell. The methods typically involve i) contacting the cell with a reporter non-covalently coupled to a ligand; ii) dissociating the reporter from the ligand and removing dissociated reporter from the surface of the cell; and iii) detecting the reporter within said cell (if any is present) where the presence of the reporter within said cell indicates that the ligand binds to an internalizing receptor and is internalized.

Abstract: The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.

Abstract: This invention provides methods, NMR probes, and NMR systems for the analysis of the contents of sealed food and beverage containers and the like.

Abstract: The present invention provides safety confirmation or hazard warning to a technician in the field, e.g., by scanning an equipment identification label and comparing the identification to a schedule of equipment inactivation times. The systems and methods of the invention can optionally provide remote interactions to reschedule inactivations or to protect the technician by lock-out from a hazardous condition.

Abstract: Stackable dollies that each include a platform component having a plurality of indentations that is structured to receive wheel components of other dollies are provided. Stackable dolly systems and related methods are also provided.

Abstract: This invention provides, e.g., a catheter with a threaded loop type snare tool for retrieval of objects from blood vessels and/or body cavities. The snare tool of the invention provides, e.g., independently maneuverable probe wires and snare wires to encircle and releasably snare foreign objects inside human and veterinary patients. The invention further provides methods of using the catheter of the invention to retrieve objects from patients.

Abstract: A method is provided for determining crystallization conditions for a protein, the method comprising: performing in a same fermentation apparatus 2 or more separate fermentations where each fermentation comprises cells expressing a protein to be purified and has a fermentation volume of less than 500 mL; purifying the expressed protein from the separate fermentations; and determining crystallization conditions for the purified protein by submicroliter crystallization experiments.

Abstract: This invention is based on the discovery that chemokines induce fibroblasts to differentiate to myofibroblasts, which play a critical role in wound healing and are implicated in a number of fibrotic diseases. This activity has been localized to a peptide in the N-terminus of several chemokines. Accordingly, the invention provides polypeptides that induce the differentiation of fibroblasts to myofibroblasts in vitro and in vivo, nucleic acids encoding such polypeptides and related vectors, host cells, and composition containing these components. The invention also encompasses methods for inducing or inhibiting differentiation of fibroblasts to myofibroblasts, in vivo as well as in vitro, and screening methods for identifying other agents that modulate myofibroblast differentiation.

Abstract: Nanostructure manufacturing methods and methods for assembling nanostructures into functional elements such as junctions, arrays and devices are provided. Systems for practicing the methods are also provided.

Abstract: The present invention provides methods of analyzing and/or displaying data. In one aspect, the invention provides methods for visualizing or displaying high dynamic range data obtained from flow cytometry analyses. Related systems and computer programs products are also provided.

Abstract: The present invention provides compositions (small molecules, oligomers and polymers) that can be used to modify charge transport across a nanocrystal surface or within a nanocrystal-containing matrix, as well as methods for making and using the novel compositions.

Abstract: This invention provides a new procedure for attaching molecules to semiconductor surfaces, in particular silicon. The molecules, which include, but are not limited to porphyrins and ferrocenes, have been previously shown to be attractive candidates for molecular-based information storage. The new attachment procedure is simple, can be completed in short times, requires minimal amounts of material, is compatible with diverse molecular functional groups, and in some instances affords unprecedented attachment motifs. These features greatly enhance the integration of the molecular materials into the processing steps that are needed to create hybrid molecular/semiconductor information storage devices.

Abstract: A computer has its programs in instructions and operand descriptors to specify the operands of the instructions. Apparatus for identifying data coherency and encaching requirements and providing access control in a computer system with operands of its instructions specified by operand descriptors is described hereby. In a computer system wherein data items (operands) are represented by operand descriptors that can comprise object numbers, addresses, data types and sizes, vector information and other relevant information concerning the operands, with one bit to identify if the data coherency is to be maintained, another bit to identify if the data is cached, and a field to provide information on the privilege of Read, Write and Execute, and Supervisor or User mode. When an operand is accessed, the respective access control code is checked to validate if any protection is violated, whether caching is activated and whether it is required to maintain data coherency.

Abstract: This invention provides novel peptides that ameliorate one or more symptoms of atherosclerosis. The peptides are highly stable and readily administered via an oral route.

Abstract: The present invention provides grasping mechanisms, gripper apparatus/systems, and related methods. Grasping mechanisms that include stops, support surfaces, and height adjusting surfaces to determine three translational axis positions of a grasped object are provided. In addition, grasping mechanisms that are resiliently coupled to other gripper apparatus components are also provided.

Abstract: A striking head-interchangeable hammer includes a handle, a head body connected securely to the handle and formed with a through-hole and upper and lower bores, a striking body having a connecting portion that is received fittingly in the lower bore, and a stress-distributable fastener including a connecting rod that extends through the through-hole and the upper and lower bores in the head body to engage threadedly the striking body, and that is formed with an abutting flange received fittingly in the upper bore and anchored on and abutting against an abutting face of a bore-defining wall of the upper bore upon tightening of the connecting rod onto the striking body.

Abstract: Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars.

Abstract: A information search and navigation system indexes, categorizes and condenses data from text or other documents. In various embodiments, the invention further can utilize user information goals, document or website types and multi-page link path options that together provide a fast, multi-page Web navigation system.