Abstract: The present invention provides a process for determining genotypes in highly polymorphic systems by polymerase chain reaction amplification of cDNA or genomic DNA and direct sequencing polymerase chain reaction products using oligonucleotide primers. More specifically, Class I HLA genotypes can be unambiguously determined in any subject in 16-24 hours by direct sequencing of HLA-A, HLA-B, and HLA-C transcripts enzymatically amplified and sequenced using a limited number of selected oligonucleotide.
Type:
Grant
Filed:
July 28, 1994
Date of Patent:
January 14, 1997
Assignee:
Regents of the University of Minnesota
Inventors:
Pedro Santamaria, Michael T. Boyce-Jacino, Jose J. Barbosa, Stephen S. Rich, Anthony J. Faras
Abstract: A method is disclosed for obtaining aroma components and aromas from their glycosidic precursors containing a .beta.-apioside with the use of .beta.-apiosidase.
Type:
Grant
Filed:
July 18, 1994
Date of Patent:
November 12, 1996
Assignees:
Gist-brocades, N.V., Institute National de la Recherche Agronomique
Inventors:
Ziya Gunata, Sylvaine Bitteur, Raymond Baumes, Jean-Marc Brillouet, Claude Tapiero, Claude Bayonove, Robert Cordonnier
Abstract: Methods and compositions are provided for maintaining pregnancy and preventing rejection of transplanted tissue. A polypeptide characterized as capable of isolation from conceptus tissue and capable of inhibiting mitogen induced lymphocyte blastogenesis is employed which is introduced into the uterus or systemically.
Abstract: Novel constructs are provided for expression of physiologically active mammalian proteins in plant cells, either in culture or under cultivation. The constructs provide a promoter functional in a plant host, a structural gene coding for mammalian protein and a terminator functional in a plant host. The construct is introduced into a plant cell to become integrated into the plant genome for expression in the plant cells or plants. The plant cells may be harvested and the mammalian protein isolated in physiologically active form.
Type:
Grant
Filed:
December 8, 1993
Date of Patent:
August 27, 1996
Assignee:
Calgene, Inc.
Inventors:
Robert M. Goodman, Vic C. Knauf, Catherine M. Houck, Luca Comai
Abstract: Methods and compositions based upon natural flavenoid aldehydes are provided, which find use as pesticides. The pesticides are formulated in a variety of ways, including dusts, sprays, shampoos and soaps, and can be bound to a solid support or provided as bait or directly impregnated into organic matter infested by or susceptible to infestation by a target pest.
Type:
Grant
Filed:
December 30, 1994
Date of Patent:
July 16, 1996
Assignee:
ProGuard, Inc.
Inventors:
Ralph W. Emerson, Bradford G. Crandall, Jr.