Patents Represented by Law Firm Reed & Robbins
  • Method of using colored phosphorylating reagents
    Patent number: 5252760
    Abstract: A method of phosphorylating a nucleoside or an oligonucleotide chain having a free 2', 3' or 5' hydroxyl moiety is provided. The method involves the use of a phosphorylating reagent which is selected such that the extent of phosphorylation can be monitored colorimetrically, easily and accurately. The phosphorylating reagent contains an aromatic species such as a dimethoxytrityl group that is cleavable with acid and colorimetrically detectable upon release.
    Type: Grant
    Filed: August 18, 1987
    Date of Patent: October 12, 1993
    Assignee: Chiron Corporation
    Inventors: Michael S. Urdea, Thomas Horn