Patents Represented by Attorney, Agent or Law Firm Richard F. Trecartin
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Patent number: 6255113Abstract: The invention relates to methods for targeting an exogenous polynucleotide or exogenous complementary polynucleotide pair to a predetermined endogenous DNA target sequence in a eukaryotic cell by homologous pairing, particularly for altering an endogenous DNA sequence, such as a chromosomal DNA sequence, typically by targeted homologous recombination. In certain embodiments, the invention relates to methods for targeting an exogenous polynucleotide having a linked chemical substituent to a predetermined endogenous DNA sequence in a metabolically active eukaryotic cell, generating a DNA sequence-specific targeting of one or more chemical substituents in an intact nucleus of a metabolically active eukaryotic cell, generally for purposes of altering a predetermined endogenous DNA sequence in the cell.Type: GrantFiled: February 8, 1995Date of Patent: July 3, 2001Assignee: SRI InternationalInventors: David A. Zarling, Elissa P. Sena
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Patent number: 6251278Abstract: Chromatographic material having the general formula S-B-X-Y-L where S is a solid support, B is a binding group, X is a substantially non-ionic hydrophilic organic spacer, Y is a coupling group and L is an affinity ligand. The chromatographic material is substantially free of non-specific adsorption and is stable at high pH.Type: GrantFiled: March 3, 1999Date of Patent: June 26, 2001Assignee: ChromatoChem, Inc.Inventor: Richard Frederick Hammen
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Patent number: 6248229Abstract: The invention relates to novel methods and compositions for the detection of analytes using the nuclear reorganization energy, &lgr;, of an electron transfer process.Type: GrantFiled: October 13, 1999Date of Patent: June 19, 2001Assignee: Clinical Micro Sensors, Inc.Inventor: Thomas J. Meade
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Patent number: 6245337Abstract: Haemophilus adhesion and penetration proteins, nucleic acids, vaccines and monoclonal antibodies are provided.Type: GrantFiled: August 25, 1994Date of Patent: June 12, 2001Assignees: Washington University, The Board of Trustees of the Leland Stanford Junior UniversityInventors: Joseph W. St. Geme, III, Stanley Falkow
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Patent number: 6238670Abstract: Described herein are compositions which modulate the immune response. In one aspect, a composition is described which comprises an antigen covalently linked to a ligand for CD21(CR2) or CD19. This antigen is not associated with a complement C3 fragment through an ester bond derived from the internal thioester of the complement C3 fragment.Type: GrantFiled: October 21, 1997Date of Patent: May 29, 2001Assignee: Cambridge University Technical Services LimitedInventors: Douglas T. Fearon, Paul W. Dempsey
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Patent number: 6232295Abstract: A delivery vehicle is described that is capable of being specifically bound to and taken into targeted cells, delivering numerous paramagnetic ions for magnetic resonance imaging (MRI) of the cells. The delivery vehicle comprises a polymeric molecule having a net positive charge complexed with another polymeric molecule having a net negative charge. Cell targeting moieties and MRI contrast agents are attached to one or both of the polymeric molecules. In one embodiment, the polymeric molecule having a net negative charge is a nucleic acid. Thus, the delivery vehicles can be used in clinical protocols in which nucleic acids for gene therapy and agents for MRI contrast are co-transported to specific cells allowing medical imaging monitoring of nucleic acid delivery.Type: GrantFiled: October 12, 1994Date of Patent: May 15, 2001Inventors: Jon Faiz Kayyem, Thomas J. Meade, Scott E. Fraser
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Patent number: 6232062Abstract: The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids with electron transfer moieties and electrodes to produce a new class of biomaterials, and to methods of making and using them.Type: GrantFiled: August 14, 1997Date of Patent: May 15, 2001Assignee: Clinical Micro Sensors, Inc.Inventors: Jon Faiz Kayyem, Stephen D. O'Connor
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Patent number: 6221583Abstract: The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids with electron transfer moieties and electrodes to produce a new class of biomaterials, and to methods of making and using them.Type: GrantFiled: July 24, 1997Date of Patent: April 24, 2001Assignee: Clinical Micro Sensors, Inc.Inventors: Jon Faiz Kayyem, Stephen D. O'Connor, Michael Gozin, Changjun Yu, Thomas J. Meade
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Patent number: 6200761Abstract: The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.Type: GrantFiled: December 6, 1999Date of Patent: March 13, 2001Assignee: California Institute of TechnologyInventors: Thomas J. Meade, Jon Faiz Kayyem, Scott E. Fraser
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Patent number: 6200578Abstract: The invention relates to novel Haemophilus adhesion proteins, nucleic acids, and antibodies.Type: GrantFiled: December 29, 1997Date of Patent: March 13, 2001Assignees: St. Louis University, Washington UniversityInventors: Joseph St. Geme, Stephen J. Barenkamp
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Patent number: 6200812Abstract: The invention relates to methods for targeting an exogenous polynucleotide or exogenous complementary polynucleotide pair to a predetermined endogenous DNA target sequence in a target cell by homologous pairing, particularly for altering an endogenous DNA sequence, such as a chromosomal DNA sequence, typically by targeted homologous recombination. In certain embodiments, the invention relates to methods for targeting an exogenous polynucleotide having a linked chemical substituent to a predetermined endogenous DNA sequence in a metabolically active target cell, generating a DNA sequence-specific targeting of one or more chemical substituents in an intact nucleus of a metabolically active target cell, generally for purposes of altering a predetermined endogenous DNA sequence in the cell.Type: GrantFiled: April 8, 1999Date of Patent: March 13, 2001Assignee: SRI InternationalInventors: Sushma Pati, David A. Zarling
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Patent number: 6197515Abstract: An article suitable for use as a biosensor includes a molecule of a formula X—R—Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.Type: GrantFiled: April 21, 1999Date of Patent: March 6, 2001Assignee: Harvard UniversityInventors: Cynthia C. Bamdad, George B. Sigal, Jack L. Strominger, George M. Whitesides
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Patent number: 6188965Abstract: The present invention relates to apparatus and methods for quantitative protein design and optimization.Type: GrantFiled: April 10, 1998Date of Patent: February 13, 2001Assignee: California Institute of TechnologyInventors: Stephen L. Mayo, Bassil I. Dahiyat, D. Benjamin Gordon, Arthur Street
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Patent number: 6184348Abstract: Novel polymers are provided which are produced by recombinant techniques. The polymers are characterized by having a small repeating sequence which provides for strands capable of associating, resulting in useful structural characteristics, where the strands are joined by turns or loops which are flexible and available for interaction with the environment. Specifically, repeating groups of naturally occurring proteins such as silk are modified by introduction of an amino-acid sequence at a site which provides for a turn between strands to provide for readily available oligopeptides capable of interacting with molecules in the environment.Type: GrantFiled: June 7, 1995Date of Patent: February 6, 2001Assignee: Protein Polymer TechnologiesInventors: Franco A. Ferrari, Joseph Cappello
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Patent number: 6180352Abstract: The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.Type: GrantFiled: December 10, 1999Date of Patent: January 30, 2001Assignee: California Insitute of TechnologyInventors: Thomas J. Meade, Jon Faiz Kayyem, Scott E. Fraser
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Patent number: 6177075Abstract: The present invention relates to an isolated small RNA virus capable of infecting insect species including Heliothis species, and to the nucleotide sequences and proteins encoded thereby. The invention contemplates uses of the virus in controlling insect attack in plants.Type: GrantFiled: June 7, 1995Date of Patent: January 23, 2001Assignee: Commonwealth Scientific and Industrial Research Organization and Pacific Seeds Pty., Ltd.Inventors: Peter Daniel Christian, Karl Hienrich Julius Gordon, Terry Nelson Hanzlik
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Patent number: 6177250Abstract: The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.Type: GrantFiled: May 7, 1999Date of Patent: January 23, 2001Assignee: California Institute of TechnologyInventors: Thomas J. Meade, Jon Faiz Kayyem, Scott E. Fraser
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Patent number: 6177243Abstract: Methods and compositions are provided for detecting nucleic acid sequences. In particular, pairs of probes are employed, where the pair defines a substantially contiguous sequence on a target nucleic acid. Each of the pairs has a side chain which forms a stem of the two side chains which non-covalently binds and is capable of forming a cross-link upon activation, when the probes and sample nucleic acid are base paired. Cross-linking of the stems when unbound to complementary DNA is inhibited. Each of the nucleic acids is initially present as single stranded nucleic acid to allow for base pairing, so that the probes bind to homologous target nucleic acid. The assay mixture is activated to provide cross-linking, the double stranded nucleic acid melted, and the process of base pairing, activation and melting repeated, a sufficient number of cycles, to provide a detectable amount of cross-linked probes.Type: GrantFiled: December 5, 1997Date of Patent: January 23, 2001Assignee: NaxcorInventors: David Albagli, Reuel VanAtta, Michael Wood
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Patent number: 6171817Abstract: Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence.Type: GrantFiled: November 24, 1997Date of Patent: January 9, 2001Assignee: Genencor International, Inc.Inventors: Randy Michael Berka, Daniel Cullen, Gregory Lawrence Gray, Kirk James Hayenga, Virgil Bryan Lawlis
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Patent number: 6090933Abstract: The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids with electron transfer moieties and electrodes to produce a new class of biomaterials, and to methods of making and using them.Type: GrantFiled: August 14, 1997Date of Patent: July 18, 2000Assignee: Clinical Micro Sensors, Inc.Inventors: Jon Faiz Kayyem, Stephen D. O'Connor, Michael Gozin, Changjun Yu, Thomas J. Meade