Abstract: The invention concerns DNA fragments derived from the genomic DNA of HPV-33. These fragments are selected from the group of fragments extending between the nucleotide extremities defined hereafter in relation to the nucleotide-numbering in FIGS. 1a and 1b respectively: 76–556 543–864 867–2811 2728–3808 3326–3575 3842–4079 4198–5611 5516–8091. The invention also relates to the use of these fragments as probes for the detection of HPV in tissue cultures.