Abstract: A method is provided for producing a population of genetically modified T cells. In the method, an in vitro population of T cells is activated by contacting said population with a CD3 binding agent. Genetic modification is then carried out with the activated T cells by contacting the same with a suitable gene transfer vector.

Abstract: Compositions are provided which include biodegradable microparticles with entrapped or adsorbed antigens, in combination with submicron oil-in-water emulsions. Also provided are methods of immunization which comprise administering to a vertebrate subject (a) a submicron oil-in-water emulsion, and (b) a therapeutically effective amount of a selected antigen entrapped in a microparticle.

Abstract: New immunological carrier systems, DNA encoding the same, and the use of these systems, are disclosed. The carrier systems include chimeric proteins which include a leukotoxin polypeptide fused to one or more selected GnRH multimers which comprise at least one repeating GnRH decapeptide sequence, or at least one repeating unit of a sequence corresponding to at least one epitope of a selected GnRH molecule. Under the invention, the selected GnRH sequences may all be the same, or may correspond to different derivatives, analogues, variants or epitopes of GnRH so long as the GnRH sequences are capable of eliciting an immune response. The leukotoxin functions to increase the immunogenicity of the GnRH multimers fused thereto.

Abstract: New subunit vaccines from Haemophilus somnus are disclosed. The vaccines include an outer membrane protein extract of H. somnus which is enriched with iron-regulated proteins. Additional antigens, such as antigens derived from Pasteurella haemolytica, can be included in the vaccine composition to provide protection against a variety of disease states.

Abstract: The invention provides for the inhibition of the activity of TFPI to prevent TFPI from countering the procoagulant activities associated with many tumors. Inhibition of TFPI activity by the method of the invention facilitates the propensity toward thrombosis in malignancies and therefore promotes tumor cell death. The method of the invention also includes, in addition to the administration of an inhibitor of TFPI, co-administration of an agent capable of inducing or augmenting in the targeted tumor the release or induction of factors that initiate or enhance clotting, such as TF and Factors VIIa and Xa.