Abstract: This invention generally relates to a reflection-photometric analytical system having a measuring head comprising a source of radiation and a radiation detector for the reflection-photometric analysis of a target surface of a test object arranged at a distance from the measuring head and in particular of a test strip for body fluids such as urine or blood. A triangulation unit operating on the basis of optical triangulation is proposed for contact free checking of the distance in order to monitor or regulate the measuring distance.

Abstract: The present invention is directed to a new composition for performing a nucleic acid amplification reaction comprising (i) a thermostable DNA-Polymerase, (ii) a thermostable 3?-5? Exonuclease, and (iii) at least one primer for nucleic acid amplification with a modified 3? terminal residue which is not elongated by said thermostable DNA-Polymerase as well as methods for performing a PCR reaction using this composition. Furthermore, the method is directed to kits comprising such a composition.

Abstract: A method is provided for the production of a bovine pancreatic protein with a specific desoxyribonuclease activity of at least 6,000 units per mg of protein. Methylotrophic yeast is used as a heterologous host organism. The bovine pancreatic protein is secreted into the growth medium from which it is purified.

Abstract: Compounds including haptens, intermediates, and immunogens that are useful in the production of antibodies specific for the methylenedioxy class of amphetamine derivatives are described. Antibodies specific for the methylenedioxy class of amphetamine derivatives, reagent kits containing antibodies specific for the methylenedioxy class of amphetamine derivatives, methods of producing antibodies specific for the methylenedioxy class of amphetamine derivatives, and methods of detecting analytes including members of the methylenedioxy class of amphetamine derivatives are also described.

Abstract: Systems, client computing devices, server computing devices, and methods are disclosed for accessing medical devices, providing remote access to medical devices and/or remotely accessing medical devices. In one exemplary embodiment, client computing devices utilize protocol components that may be obtained from a server computing device via a network to communicate with medical devices in a communications protocol supported by the medical device.

Abstract: The present invention comprises novel analogs of ecstasy-class compounds and novel ecstasy-class immunogens leashed out of, i.e., derived from, the methylenedioxy position. The invention also comprises unique monoclonal antibodies generated using MDO-leashed MDMA immunogens as well as unique conjugates and tracers. These antibodies, conjugates, and tracers are useful in immunoassays for the detection of ecstasy-class compounds in biological fluids.

Abstract: The present invention relates to compounds and more particularly to compounds which are useful for the determination of bicarbonate and the delivery of bicarbonate to a non-aqueous solution. The invention further relates to an optical sensor for the determination of bicarbonate in a liquid sample.

Abstract: A water-soluble reference standard is useful for immunoassays of a lipophilic drugs. The reference standard is a compound of formula (I): G-(L)n—Y;??(I) where G is a lipophilic drug; L is a linker which is an alkyl group or heteroalkyl group containing from 1 to 20 carbon atoms; n is 0 or 1; and Y is a water-solubilizing group such as —SO3?, —NR—SO3?, —P(?O)(OH)(O?), or —O—P(?O)(OH)(O?); where R is H or an alkyl group of 1 to 10 carbon atoms.

Abstract: The present invention concerns a method for improving the stability of linear short DNA towards exonucleases in cell-free in vitro transcription/translation systems using lysates containing exonucleases or in cellular systems containing exonucleases, wherein the stability of the linear short DNA is improved by adding unspecific linear DNA.

Abstract: Analogs of saquinavir functionalized at the quinoline portion of the molecule are described. These include pyridyl analogs (replacing the quinoline ring) with a functional handle out of the ring allowing for elaboration with linkers terminated by a functional group such as an activated ester which are useful for attaching the molecule to other entities such as proteins, polysaccharides, and the like. Analogs of saquinavir derivatized out of the quinoline ring are also described.

Abstract: Assembly and method for measuring the concentration of an analyte in a biological matrix. The assembly includes an implantable optical-sensing element that comprises a body, and a membrane mounted on the body in a manner such that the membrane and the body define a cavity. The membrane is permeable to the analyte, but is impermeable to background species in the biological matrix. A refractive element is positioned in the cavity. A light source transmits light of a first intensity onto the refractive element, and a light detector receives light of a second intensity that is reflected from the cavity. A controller device optically coupled to the detector compares the first and second light intensities, and relates the intensities to analyte concentration.

Abstract: The invention relates to a method for determining the current filling level of a liquid, preferably a calibrating, quality control, or cleaning fluid, or waste water, in a container, for example of an analyzer, where an immersion pipe is dipped into the liquid in the container to be filled or drained. The immersion pipe is connected to a pumping device via a tube system. The pressure p in the tube system is measured and increased, starting from the ambient pressure p0, until a first discontinuous pressure change occurs at pressure p1 due to the liquid column in the immersion pipe being forced out of the pipe, and/or the pressure p in the tube system is decreased until the liquid column in the immersion pipe reaches a change in cross-section of the immersion pipe, which change is situated above the maximum filling level of the liquid, such that a second discontinuous pressure change occurs at pressure p2 due to the liquid column reaching the change in cross-section.

Abstract: The present invention concerns a method for the expression of target proteins in in vitro translation systems, characterized in that folding helper proteins are co-expressed in this system. The co-expressed folding helper proteins are selected from one or several of the following protein classes: Hsp70, Hsp60, Hsp90, Hsp100 protein family, the family of small heat shock proteins and isomerases.

Abstract: Liquid crystal display with conductive segments for representing symbols which also has at least one inverse segment that fills at least in part the display area that is not covered by the remaining segments. This liquid crystal display allows a simple, visual detection of faulty segments. For this purpose a check is carried out in which all segments and all existing inverse segments are activated which results in a homogenous image when the display is completely functional or the faulty segments are inversely displayed in relation to the remaining display surfaces.

Abstract: The heterologous expression of the reverse transcriptase from the Avian Myeloblastosis Virus (AMV-RT) in prokaryotic cells and in particular Escherichia coli (E. coli) is described in the present invention. The invention also includes certain measures to simplify the purification of the heterodimeric AMV-RT.

Abstract: A method of treating particles to be used in immunoassays reduces interference in particle agglutination assays. For particles having covalently bound antibodies and residual NHS-ester or sNHS-ester groups on the surface, the reactive esters are treated with an aqueous mixture containing an amine compound of formula (I): H2N—R—X??(I). The moiety —X is —NH2, —OH, or —CO2CH2CH3; and R is an alkyl group or an alkyl ether group. When —X is —NH2 or —CO2CH2CH3, R contains from 1 to 20 carbon atoms; and when —X is —OH, R contains from 4 to 20 carbon atoms.

Abstract: This invention involves a process for selecting and producing eukaryotic alkaline phosphatase in yeast. Yeast cells are subjected to multiple transformations using a vector comprising a first resistance marker gene and the alkaline phosphatase gene. Those strains that grow in media containing the first resistance marker are further transformed using a vector comprising a second selection marker gene and the alkaline phosphatase gene. Transformants that grow in media containing the second selection marker are selected for expressing the eukaryotic alkaline phosphatase.

Abstract: The present invention concerns a labelling reagent in which the label is bound via an amide bond and a linker to a residue of the molecule which is essentially characterized in that the N atom of the amide bond and the label are linked together directly by a covalent bond. In particular these are phosphoramidites or reactive supports suitable for nucleic acid synthesis. The invention also concerns processes for the production of such supports from suitable precursors.

Abstract: The present invention generally relates to a method for producing biosensors and a biosensor for determination of creatinine. The biosensor comprises at least two enzymes, for the amperometric determination of enzymatically degradable substances in biological liquids, the enzymes being immobilized on a working electrode.