Abstract: The functional analysis of genes frequently requires the manipulation of large genomic regions. A yeast-bacteria shuttle vector is described that can be used to clone large regions of DNA by homologous recombination. Also described is a method for isolating entire genomes, including chloroplast genomes, or large portions thereof, and manipulating the same. Also described are methods for determining minimal genomes, minimal pathway requirements, and minimal organelle genomes.
Type:
Grant
Filed:
October 6, 2008
Date of Patent:
November 20, 2012
Assignee:
Sapphire Energy, Inc.
Inventors:
Michael Mendez, Bryan O'Neill, Kari Mikkelson
Abstract: Disclosed herein are methods and processes for the recovery of oleaginous compounds from biomass and in particular biomass comprises photosynthetic microorganisms. Also disclosure are oleaginous compounds obtained using the disclosed methods.
Type:
Grant
Filed:
July 26, 2011
Date of Patent:
June 5, 2012
Assignee:
Sapphire Energy, Inc.
Inventors:
Richard J. Cranford, Alex M. Aravanis, Stilianos G. Roussis