Patents Represented by Attorney, Agent or Law Firm Sean M. Brennan
  • Regulation of endogenous gene expression in cells using zinc finger proteins
    Patent number: 6824978
    Abstract: The present invention provides methods for modulating expression of endogenous cellular genes using recombinant zinc finger proteins.
    Type: Grant
    Filed: November 3, 2000
    Date of Patent: November 30, 2004
    Assignee: Sangamo Biosciences, Inc.
    Inventors: George Norbert Cox, III, Casey Christopher Case, Stephen P. Eisenberg, Eric Edward Jarvis, Sharon Kaye Spratt
  • Gene identification
    Patent number: 6780590
    Abstract: The present disclosure provides methods and compositions for identifying a particular genomic sequence as a gene and/or a coding region, once that sequence has been tentatively identified as a gene based on genomic analysis using one or more gene prediction algorithms. The methods include the use of exogenous molecules such as zinc finger proteins which are capable of binding to and modulating expression of gene transcription, targeted to putative gene sequences, followed by assay for one or more selected phenotypes.
    Type: Grant
    Filed: August 28, 2001
    Date of Patent: August 24, 2004
    Assignee: Sangamo Biosciences, Inc.
    Inventors: Casey C. Case, Fyodor Urnov
  • Functional genomics using zinc finger proteins
    Patent number: 6777185
    Abstract: The present invention provides methods of regulating gene expression using recombinant zinc finger proteins, for functional genomics and target validation applications.
    Type: Grant
    Filed: August 9, 2001
    Date of Patent: August 17, 2004
    Assignee: Sangamo Biosciences, Inc.
    Inventors: Casey C. Case, Lei Zhang, Fyodor Urnov
  • Screening system for zinc finger polypeptides for a desired binding ability
    Patent number: 6733970
    Abstract: This invention relates to a method for producing a zinc finger nucleic acid binding protein comprising preparing a zinc finger protein according design rules, varying the protein at one or more positions, and selecting variants which bind to a target nucleic acid sequence by polysome display.
    Type: Grant
    Filed: May 8, 2001
    Date of Patent: May 11, 2004
    Assignee: Gendaq Limited
    Inventors: Yen Choo, Michael Moore
  • Cells for drug discovery
    Patent number: 6689558
    Abstract: Disclosed herein are compositions and method useful in screening a compound for its interaction and/or effect with a molecular target and/or cellular process.
    Type: Grant
    Filed: February 8, 2001
    Date of Patent: February 10, 2004
    Assignee: Sangamo BioSciences, Inc.
    Inventor: Casey Case
  • Pharmacogenomics and identification of drug targets by reconstruction of signal transduction pathways based on sequences of accessible regions
    Patent number: 6610489
    Abstract: Methods for the identification of a drug target, wherein the drug target is a component of a signal transduction pathway, are provided. The methods are based, in part, on selection of a gene of therapeutic interest and determination of regulatory DNA sequences which regulate expression of the gene in a cell of interest, followed by reconstruction of signal transduction pathways which act on molecules which bind to the regulatory sequence. Also provided are methods for assessing potential pharmacological effects and/or side effects of an agent, based on the effect of the agent on the distribution and properties of accessible regions in cellular chromatin.
    Type: Grant
    Filed: April 27, 2001
    Date of Patent: August 26, 2003
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Alan Wolffe, Fyodor Urnov, Dmitry Guschin, Trevor Collingwood, Xiao-Yong Li, Brian Johnstone
  • Regulation of endogenous gene expression in cells using zinc finger proteins
    Patent number: 6607882
    Abstract: The present invention provides methods for modulating expression of endogenous cellular genes using recombinant zinc finger proteins.
    Type: Grant
    Filed: January 6, 2000
    Date of Patent: August 19, 2003
    Assignee: Sangamo BioSciences, Inc.
    Inventors: George N. Cox, III, Casey C. Case, Stephen P. Eisenberg, Eric E. Jarvis, Sharon K. Spratt
  • Methods for designing exogenous regulatory molecules
    Patent number: 6511808
    Abstract: Methods for the design of exogenous regulatory molecules, comprising a DNA-binding domain and a functional domain, are provided. The methods rely on the identification, isolation and characterization of regulatory DNA sequences obtained on the basis of their accessibility in cellular chromatin.
    Type: Grant
    Filed: April 27, 2001
    Date of Patent: January 28, 2003
    Assignee: Sangamo Biosciences, Inc.
    Inventors: Alan Wolffe, Fyodor Urnov, Dmitry Guschin, Trevor Collingwood, Xiao-Yong Li, Brian Johnstone
  • Methods of using randomized libraries of zinc finger proteins for the identification of gene function
    Patent number: 6503717
    Abstract: The present invention relates to methods of using libraries of randomized zinc finger proteins to identify genes associated with selected phenotypes.
    Type: Grant
    Filed: December 6, 2000
    Date of Patent: January 7, 2003
    Assignee: Sangamo Biosciences, Inc.
    Inventors: Casey C. Case, Qiang Liu, Edward J. Rebar, Alan P. Wolffe
  • Zinc finger polypeptides capable of binding DNA quadruplexes
    Patent number: 6492117
    Abstract: The present invention relates to isolated or purified molecule(s) capable of binding to one or more of telomeric, G-quadruplex, or G-quartet nucleic acid(s).
    Type: Grant
    Filed: July 12, 2000
    Date of Patent: December 10, 2002
    Assignee: Gendaq Limited
    Inventors: Yen Choo, Mark Isalan, Sachin D. Patel, Shhankar Balasubramanian, Xiaohai Liu
  • Poly zinc finger proteins with improved linkers
    Patent number: 6479626
    Abstract: Provided herein are chimeric zinc finger proteins comprising flexible linkers of at least 6 amino acids in length. Also provided are methods of making and using these chimeric zinc finger proteins.
    Type: Grant
    Filed: March 1, 1999
    Date of Patent: November 12, 2002
    Assignee: Massachusetts Institute of Technology
    Inventors: Jin-Soo Kim, Carl O. Pabo
  • General strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites
    Patent number: 6410248
    Abstract: The present invention provides methods for making zinc finger proteins with high affinity for their targets, by sequentially selecting each finger of the protein.
    Type: Grant
    Filed: January 29, 1999
    Date of Patent: June 25, 2002
    Assignee: Massachusetts Institute of Technology
    Inventors: Harvey A. Greisman, Carl O. Pabo