Abstract: A fusion gene is provided comprising the cDNA of Renilla luciferase and the cDNA of the "humanized" Aequorea green fluorescent protein. The fusion gene was used to produce a novel protein, the "Renilla-GFP fusion protein," which displayed both the luciferase activity of Renilla luciferase, and the green fluorescence of GFP. The Renilla-GFP fusion gene is useful as a double marker for monitoring gene expression quantitatively in UV light and by enzyme activity.
Type:
Grant
Filed:
December 23, 1996
Date of Patent:
November 2, 1999
Assignee:
Loma Linda University
Inventors:
Aladar A. Szalay, Gefu Wang, Yubao Wang
Abstract: A seat for a substantially cylindrically-shaped pail having a pail opening, a pail lip adjacent the opening, and a hollow compartment inside the pail is disclosed. The seat comprises a platform for seating a person when the seat is mounted on the pail and grooves formed by walls on opposite sides of the platform. The grooves fit on the pail lip when the seat is in use. The seat has a width substantially less than a diameter of the pail opening so that a person seated on the platform can reach between the platform and the pail lip to remove or insert hand tools. The seat also fits into the pail when not in use. The seat has ribs on its bottom side for strengthening purposes.
Abstract: A process for electroelution of a gel containing charged macromolecules, such as proteins or polynucleotides, comprising the steps of providing a plurality of adjacent parallel chambers having a trapezoidal cross-section, placing a gel containing the charged macromolecules onto first open sides of the chambers, placing a semipermeable membrane onto second open sides of the chambers, filling the chambers with an elution buffer and applying a voltage difference across the chambers so that charged macromolecules in the gel migrate into the elution buffers in the chambers. Also, an apparatus for electroelution of a gel containing charged macromolecules having, preferably, a plurality of adjacent parallel chambers having a trapezoidal cross-section and vents for removing the product without disassembling the apparatus.
Abstract: A method of repairing damaged hot refractory linings in high-temperature vessels includes forming an opening through the outer shell of the vessel proximate to the damaged region of the hot refractory lining, and introducing a refractory material into the damaged region. The refractory material is rapidly injected in a sufficient amount to effect complete filling, and is fast curing to strength at the operating temperature of the vessel. The method eliminates the need to allow the vessel to cool down to ambient temperature and then reheat the vessel to operating temperature thereby eliminating thermal cycling of the refractory lining.
Abstract: A gas burner assembly is provided which can be used in an unvented gas-fired fireplace and which closely approximates the look of a wood-fired fireplace. The gas burner assembly has a gas distribution chamber with a horizontal base, a vertical backwall, a top wall, a pair of opposing vertical side walls and a front wall. The front wall is a continuous surface having a vertical forward section and a substantially concave rear section. A small number of ports are disposed within the front wall to generate a small, evenly-distributed flame pattern across the front wall. A flame deflector plate located forward of the front wall to direct flame from the lower portion of the front wall upwardly. A large number of ports are disposed within the top wall to generate a large vertical flame pattern at the top wall.
Abstract: Thermoplastic elastomers (TPEs) containing side chain crystalline (SCC) blocks. The SCC blocks my be hard (A) blocks or the soft (B) blocks (or both) in the TPE. Some of these TPEs are novel, e.g. those in which A blocks are SCC blocks, and the B blocks are polyethers, polyacrylates, polyamides, polyurethanes or polysiloxanes. The SCC-containing TPEs are particularly useful as matrix materials for other components which are dispersed therein, e.g. energetic solids and other thermally responsive materials.
Type:
Grant
Filed:
April 14, 1993
Date of Patent:
September 9, 1997
Assignee:
Landec Corporation
Inventors:
Steven P. Bitler, Ray F. Stewart, David A. Kamp, Robert G. Freelin, Valentine Y. Yoon
Abstract: A protective garment for the hip, including the hip joint, that comprises a pair of loose fitting or form-fitting shorts having an upper right lateral section, a lower right lateral section, an upper left lateral section, a lower left lateral section and a lower back section. The garment fruther comprises protective padding in combination with one or more of these section. The padding in combination with the upper right lateral section is separated from the padding in combination with the lower right lateral section by a first gap, and the padding in combination with the upper left lateral section is separated from the padding in combination with the lower left lateral section by a second gap. The first and second gaps comprise substantially unpadded flexible material thereby allowing substantially unhindered flexion and abduction of the hip joint. The padding can be compartmentalized or noncompartmentalized and can be in a variety of configurations.
Abstract: A golf club aligning aid is provided which allows a golfer to quickly improve his or her golf swing. The aligning aid can be a small strip of flexible material which can be disposed with a planar "flag" portion which is disposed perpendicular to the shaft of the club. This planar portion can be rotated about the shaft for proper alignment with respect to the leading edge of the face of the golf club head. Preferably, the aligning aid is attached on or near the handle of the club by being snugly wrapped around the shaft and attached to itself, such as by a hook and loop fastener. In one embodiment, the aligning aid also has a graphical design disposed on one end of the planar portion. The graphical design has a first axis which, when the aid is attached to the clubs is perpendicular to the shaft of the golf club and a second axis designed to be disposed parallel to the shaft of the club. The ratio of the length of the second axis to the length of the first axis is between about 0.25 and about 0.75.