Abstract: Disclosed is an autoreplicative plasmid containing DNA which encodes herbicide resistance, such that the plasmid is capable of transforming a yeast cell to become resistant to one or more organic herbicide growth inhibitors. Also disclosed is a process for the selective culture of yeasts transformed by the plasmid.
Type:
Grant
Filed:
May 8, 1984
Date of Patent:
May 17, 1988
Assignee:
Labofina, S.A.
Inventors:
Jean M. A. G. Delcour, Anne-Marie J. C. G. Colson-Corbisier, Annie F. J. De Baetselier-van Broekhoven, Charles Colson
Abstract: Vectors are disclosed for cloning any foreign DNA in prokaryotic and eukaryotic organisms, which vectors comprise a restriction site bank containing a large number of unique restriction sites. The disclosed vectors permit the cloning of large DNA fragments and complete genes without the risk of unwanted DNA cleavage by restriction enzymes. Also disclosed are methods for preparing the aforementioned vectors and processes using the vectors for the cloning of DNA, for the production of polypeptides, and the like.