Patents Represented by Attorney Stephen C. Macevicz
  • Patent number: 5653259
    Abstract: A manifold block and valving system using the manifold block are provided, which are used for conducting chemical reagents, solvents, and other fluids. The manifold block includes a "straight-through" common passage in fluid connection with several entry ports, including also a number of projections in the common passage in the vicinity of the entry ports. These projections partially obstruct the fluid flow, causing a turbulence that provides a washing action in those port regions. The manifold may be constructed of a photosensitive glass ceramic such as Fotoceram.TM. which consists of a number of layers which have been processed to form the manifold block, or of other suitable materials such as silicon.
    Type: Grant
    Filed: October 6, 1995
    Date of Patent: August 5, 1997
    Assignee: Applied Biosystems, Inc.
    Inventor: Paul O. Ramstad
  • Patent number: 5646267
    Abstract: Methods and compounds are provided for solid phase synthesis of oligonucleotides and related polymers by condensing protected monomer-O-[1,3,2-dichalcogen-substituted-phospholane] synthons in the presence of a catalytic base. Compounds of the invention include 2-N-substituted-1,3,2-dichalcogen-substituted-phospholane precursors of the above synthons, the protected monomer-O-[1,3,2-dichalcogen-substituted-phospholane] synthons, and P-chiral oligonucleotides and related P-chiral polymers.
    Type: Grant
    Filed: June 5, 1995
    Date of Patent: July 8, 1997
    Assignee: Polish Academy of Sciences
    Inventors: Wojciech J. Stec, Andrzej Grajkowski, Bogdan Uznanski
  • Patent number: 5639605
    Abstract: Nucleic acids encoding the .alpha. chain of the human interleukin-3 (IL-3) receptor, as well as the .alpha. chain itself, are provided. The .alpha. chain may be expressed with the .beta. chain in cellular hosts to form compositions useful in screening agonists and antagonists of human IL-3.
    Type: Grant
    Filed: December 3, 1993
    Date of Patent: June 17, 1997
    Assignee: Schering Corporation
    Inventors: Toshio Kitamura, Atsushi Miyajima
  • Patent number: 5635400
    Abstract: The invention provides a method of tracking, identifying, and/or sorting classes or subpopulations of molecules by the use of oligonucleotide tags. Oligonucleotide tags of the invention each consist of a plurality of subunits 3 to 6 nucleotides in length selected from a minimally cross-hybridizing set. A subunit of a minimally cross-hybridizing set forms a duplex or triplex having two or more mismatches with the complement of any other subunit of the same set. The number of oligonucleotide tags available in a particular embodiment depends on the number of subunits per tag and on the length of the subunit. An important aspect of the invention is the use of the oligonucleotide tags for sorting polynucleotides by specifically hybridizing tags attached to the polynucleotides to their complements on solid phase supports.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: June 3, 1997
    Assignee: Spectragen, Inc.
    Inventor: Sydney Brenner
  • Patent number: 5627155
    Abstract: BCRF1 proteins are provided for treating conditions associated with excessive production of IFN-.gamma.. Also provided are expression vectors for producing BCRF1 proteins. Compositions of the invention are useful in treating a variety of disorders, including allergy, psoriasis, tissue rejection, and MHC-linked autoimmune diseases.
    Type: Grant
    Filed: May 26, 1995
    Date of Patent: May 6, 1997
    Assignee: Schering Corporation
    Inventors: Kevin W. Moore, Robert A. Kastelein
  • Patent number: 5624800
    Abstract: Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.
    Type: Grant
    Filed: May 19, 1995
    Date of Patent: April 29, 1997
    Assignee: The Perkin-Elmer Corporation
    Inventors: Paul D. Grossman, Steven Fung, Steven M. Menchen, Sam L. Woo, Emily S. Winn-Deen
  • Patent number: 5604097
    Abstract: The invention provides a method of tracking, identifying, and/or sorting classes or subpopulations of molecules by the use of oligonucleotide tags. Oligonucleotide tags of the invention each consist of a plurality of subunits 3 to 6 nucleotides in length selected from a minimally cross-hybridizing set. A subunit of a minimally cross-hybridizing set forms a duplex or triplex having two or more mismatches with the complement of any other subunit of the same set. The number of oligonucleotide tags available in a particular embodiment depends on the number of subunits per tag and on the length of the subunit. An important aspect of the invention is the use of the oligonucleotide tags for sorting polynucleotides by specifically hybridizing tags attached to the polynucleotides to their complements on solid phase supports.
    Type: Grant
    Filed: December 19, 1994
    Date of Patent: February 18, 1997
    Assignee: Spectragen, Inc.
    Inventor: Sydney Brenner
  • Patent number: 5599675
    Abstract: The invention provides a method of nucleic acid sequence analysis based on repeated cycles of ligation to and cleavage of probes at the terminus of a target polynucleotide. At each such cycle one or more terminal nucleotides are identified and one or more nucleotides are removed from the end of the target polynucleotide, such that further cycles of ligation and cleavage can take place. At each cycle the target sequence is shortened by one or more nucleotides until the nucleotide sequence of the target polynucleotide is determined. The method obviates electrophoretic separation of similarly sized DNA fragments and eliminates the difficulties associated with the detection and analysis of spatially overlapping bands of DNA fragments in a gel, or like medium. The invention further obviates the need to generate DNA fragments from long single stranded templates with a DNA polymerase.
    Type: Grant
    Filed: March 24, 1995
    Date of Patent: February 4, 1997
    Assignee: Spectragen, Inc.
    Inventor: Sydney Brenner
  • Patent number: 5580732
    Abstract: Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieiving medium.
    Type: Grant
    Filed: August 26, 1994
    Date of Patent: December 3, 1996
    Assignee: The Perkin Elmer Corporation
    Inventors: Paul D. Grossman, Steven Fung, Steven M. Menchen, Sam L. Woo, Emily S. Winn-Deen
  • Patent number: 5552278
    Abstract: The invention provides a method of nucleic acid sequence analysis based on repeated cycles of ligation to and cleavage of probes at the terminus of a target polynucleotide. At each such cycle one or more terminal nucleotides are identified and one or more nucleotides are removed from the end of the target polynucleotide, such that further cycles of ligation and cleavage can take place. At each cycle the target sequence is shortened by one or more nucleotides until the nucleotide sequence of the target polynucleotide is determined. The method obviates electrophoretic separation of similarly sized DNA fragments and eliminates the difficulties associated with the detection and analysis of spacially overlapping bands of DNA fragments in a gel, or like medium. The invention further obviates the need to generate DNA fragments from long single stranded templates with a DNA polymerase.
    Type: Grant
    Filed: July 25, 1994
    Date of Patent: September 3, 1996
    Assignee: Spectragen, Inc.
    Inventor: Sydney Brenner
  • Patent number: 5541063
    Abstract: The present invention relates to the isolation and cloning of the .alpha.-chain of the human IL-3 receptor, which, when expressed together with the .beta.-chain of the human IL-3 receptor, forms a high affinity receptor for human IL-3. The invention further relates to a method for detecting agonists and antagonists of human IL-3 by the use of a cellular host expressing genes for the .alpha.- and .beta.-chains of the human IL-3 receptor.
    Type: Grant
    Filed: October 13, 1993
    Date of Patent: July 30, 1996
    Assignee: Schering Corporation
    Inventors: Toshio Kitamura, Atsushi Miyajima
  • Patent number: 5512668
    Abstract: Methods and compounds are provided for solid phase synthesis of oligonucleotides and related polymers by condensing protected monomer-O- 1,3,2-dichalcogen-substituted-phospholane! synthons in the presence of a catalytic base. Compounds of the invention include 2-N-substituted-1,3,2-dichalcogen-substituted-phospholane precursors of the above synthons, the protected monomer-O- 1,3,2-dichalcogen-substituted-phospholane! synthons, and P-chiral oligonucleotides and related P-chiral polymers.
    Type: Grant
    Filed: January 23, 1992
    Date of Patent: April 30, 1996
    Assignee: Polish Academy of Sciences
    Inventors: Wojciech J. Stec, Andrzej Grajkowski, Bogdan Uznanski
  • Patent number: 5489507
    Abstract: A method of detecting a target DNA in a sample is provided which includes simultaneously amplifying the target DNA and one or more internal standard DNAs, labeling the target DNA with a first color-producing or color-absorbing label, and labeling each of the one or more internal standard DNAs with a different second color-producing or color-absorbing label, the first and second color-producing and color-absorbing labels being selected so that upon illumination of the sample a first color signal is produced whenever the target DNA is present and a second color signal is produced whenever the target DNA is absent.
    Type: Grant
    Filed: May 1, 1991
    Date of Patent: February 6, 1996
    Assignee: Perkin-Elmer Corporation
    Inventor: Farid F. Chehab
  • Patent number: 5468843
    Abstract: A method is provided for C-terminal sequencing of a protein or peptide. An important feature of the method is the formation of an oxazolone moiety at the C-terminus of a protein or peptide by treatment with acetic anhydride under basic conditions followed by conversion of the oxazolone to a thiohydantoin moiety by treatment with thiocyanate under acidic conditions. Yields of thiohydantoin are further enhanced by delivering thiocyanate as the conjugate acid of a sterically hindered alkylammnonium cation.
    Type: Grant
    Filed: September 8, 1994
    Date of Patent: November 21, 1995
    Assignee: Perkin-Elmer
    Inventors: Victoria L. Boyd, MeriLisa Bozzini, G. Marc Loudon
  • Patent number: 5366860
    Abstract: A spectrally resolvable set of rhodamine dyes are provided for use in the chain termination method of nucleic acid sequencing. A different rhodamine dye from the group consisting of tetramethylrhodamine, rhodamine X, rhodamine 6G, and rhodamine 110 is attached to the base of each of the dideoxynucleotides used in the sequencing method by way of an alkynylamino linker. Preferably, the labeled dideoxynucleotides are incorporated into the growing DNA chains by Taq DNA polymerase.
    Type: Grant
    Filed: September 29, 1989
    Date of Patent: November 22, 1994
    Assignee: Applied Biosystems, Inc.
    Inventors: B. John Bergot, Vergine Chakerian, Charles R. Connell, J. Scott Eadie, Steven Fung, N. Davis Hershey, Linda G. Lee, Steven M. Menchen, Sam L. Woo
  • Patent number: 5359052
    Abstract: Methods and compounds are provided for solid phase synthesis of oligonucleotides and related polymers by condensing protected monomer-O-[1,3,2-dichalcogen-substituted-phospholane] synthons in the presence of a catalytic base. Compounds of the invention include 2-N-substituted-1,3,2-dichalcogen-substituted-phospholane precursors of the above synthons, the protected monomer-O-[1,3,2-dichalcogen-substituted-phospholane] synthons, and P-chiral oligonucleotides and related P-chiral polymers.
    Type: Grant
    Filed: May 12, 1992
    Date of Patent: October 25, 1994
    Assignee: Polish Academy of Sciences
    Inventors: Wojciech J. Stee, Andrzej Grajkowski, Bogdan Uznanski
  • Patent number: 5292875
    Abstract: A method for synthesizing sulfurized oligonucleotide analogs, such as phosphorothioate and phosphorodithioate analogs, is provided that employs a thiophosphorus compound, such as a thiophosphoric, dithiophosphoric, thiophosphinic, or dithiophosphinic acid disulfide or polysulfide, as a sulfurizing agent. The method of the invention may be used to sulfurize any phosphorous(III)containing intermediate. Preferably, the method is practiced on a commercial DNA synthesizer using phosophoramidite and/or phosphorthioamidite intermediates.
    Type: Grant
    Filed: August 27, 1993
    Date of Patent: March 8, 1994
    Assignee: Lynx Therapeutics, Inc.
    Inventors: Wojciech J. Stec, Bogdan Uznanski, B. John Bergot, Bernard L. Hirschbein, Karen L. Fearson
  • Patent number: 5281701
    Abstract: A method and compositions are provided for synthesizing polynucleotides wherein the exocyclic amino groups of 5'-O-protected-2'O-alkylsilyl-adenosine phosphoramidite and 5'-O-protected-2'-O-alkylsilylguanosine phosphoramidite monomers are protected with dialkylformamidine. In a preferred embodiment, the ribonucleoside phosphoramidite monomers are activated with ethylthiotetrazole.
    Type: Grant
    Filed: July 12, 1991
    Date of Patent: January 25, 1994
    Assignee: Applied Biosystems, Inc.
    Inventor: Ravi S. Vinayak
  • Patent number: 5262530
    Abstract: A method and system for polynucleotide synthesis are provided which employ solid phase synthesis on a nonswellable porous polystyrene support by phosphoramidite or hydrogen phosphonate chemistries. The polystyrene support gives rise to fewer tritylated failure sequences caused by chain growth from extraneous support sites, and allows lower amounts of monomer reactants to be used to achieve equal or better coupling efficiencies as those achieveable with CPG. The method and system also employ nucleoside intermediates whose exocyclic amines are protected by base-labile groups which permit simultaneous cleavage and deprotection of the completed polynucleotide chain in the presence of the solid phase support. This latter feature allows practical automation of both the synthesis and purification of polynucleotides.
    Type: Grant
    Filed: July 27, 1990
    Date of Patent: November 16, 1993
    Assignee: Applied Biosystems, Inc.
    Inventors: William A. Andrus, Christie D. McCollum, Gerald Zon
  • Patent number: 5258538
    Abstract: The compounds of the invention include novel linking agents comprising 2-substituted-3-protected-1,3,2-oxazaphosphacycloalkanes and their phosphoramidite precursors. The compounds of the invention further include conjugates of the above linking agents with oligonucleotides and polymer supports. The compounds of the present invention are useful for linking organic moieties, such as fluorescent or chromogenic dyes, to polymer supports and oligonucleotides, particularly single- and double-stranded DNA and RNA fragments.
    Type: Grant
    Filed: October 29, 1991
    Date of Patent: November 2, 1993
    Assignee: Applied Biosystems, Inc.
    Inventors: Steven Fung, Sam L. Woo, Lloyd M. Smith