Patents Represented by Attorney, Agent or Law Firm Steven R. Lazar
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Patent number: 6287857Abstract: This invention describes a nucleic acid delivery vehicle construct for transfecting and/or infecting a target cell. The construct is made of a delivery vehicle and a bifunctional complex for linking the delivery vehicle to a target cell. The bifunctional complex has a delivery vehicle-binding molecule or fragment (“delivery vehicle-binding portion”), a molecule or fragment thereof that binds to a cell surface molecule on the target cell (“cell surface molecule-binding portion”) and a linker which connects the molecules or fragments.Type: GrantFiled: October 25, 1999Date of Patent: September 11, 2001Assignee: Genzyme CorporationInventors: Catherine R. O'Riordan, Samuel C. Wadsworth
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Patent number: 6284872Abstract: Compositions of proteins with tendon/ligament-like tissue inducing activity are disclosed. The compositions are useful in the treatment of tendinitis and tendon or ligament defects and in related tissue repair.Type: GrantFiled: February 28, 1997Date of Patent: September 4, 2001Assignees: Genetics Institute, Inc., President and Fellows of Harvard CollegeInventors: Anthony J. Celeste, John M. Wozney, Vicki A. Rosen, Neil M. Wolfman, Gerald H. Thomsen, Douglas A. Melton
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Patent number: 6214586Abstract: A method is described for purifying plasmid DNA from a mixture containing plasmid DNA and genomic DNA. A solution containing both plasmid DNA and genomic DNA is treated with at least 80% by weight saturation ammonium sulfate, thereby precipitating the genomic DNA and providing purified plasmid DNA in solution. Genomic DNA levels in the purified plasmid DNA product are less than 1% by weight based on the plasmid DNA. The purified plasmid DNA is suitable for use in humans.Type: GrantFiled: December 8, 1997Date of Patent: April 10, 2001Assignee: Genzyme CorporationInventor: David S. McNeilly
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Patent number: 6143524Abstract: Provided is a fusion molecule comprising a DNA sequence encoding a thioredoxin-like protein fused to a DNA sequence encoding a second peptide or protein. The peptide or protein may be fused to the amino terminus of the thioredoxin-like molecule, the carboxyl terminus of the thioredoxin-like molecule, or within the thioredoxin-like molecule, for example at the active-site loop of the molecule. The fusion molecule may be modified to introduce one or more metal-binding/chelating amino-acid residues to aid in purification. Expression of this fusion molecule under the control of a regulatory sequence capable of directing its expression in a desired host cell, produces high levels of stable and soluble fusion protein. The fusion protein, located in the bacterial cytoplasm, may be selectively released from the cell by osmotic shock or freeze/thaw procedures. It may be optionally cleaved to liberate the soluble, correctly folded heterologous protein from the thioredoxin-like portion.Type: GrantFiled: March 4, 1997Date of Patent: November 7, 2000Assignee: Genetics Institute, Inc.Inventors: John McCoy, Elizabeth DiBlasio-Smith, Kathleen Grant, Edward R. LaVallie
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Patent number: 6143548Abstract: The present invention relates to the purification of large scale quantities of active (infectious) adenovirus and AAV, especially for use in therapeutic applications. In particular, the invention provides improved methods for contacting such viruses with suitable chromatographic materials in a fashion such that any damage to the virus, particularly to surface components thereof, resulting from contact with such chromatographic materials is minimized or eliminated. The result is the ability to rapidly and efficiently purify commercial level quantities of active (infectious) virus suitable for use in therapeutic applications, e.g. gene transfer/therapy procedures.Type: GrantFiled: June 29, 1998Date of Patent: November 7, 2000Assignee: Genzyme CorporationInventors: Catherine E. O'Riordan, Amy E. Erickson, Alan E. Smith
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Patent number: 6066317Abstract: A novel mammalian cytokine, IL-11, and processes for producing it are disclosed. IL-11 may be used in pharmaceutical preparations for stimulating and/or enhancing cells involved in the immune response and cells involved in the proper functioning of the hematopoietic system.Type: GrantFiled: July 24, 1998Date of Patent: May 23, 2000Assignee: Genetics Institute, Inc.Inventors: Yu-Chung Yang, Frances K. Bennett, Stephan R. Paul
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Patent number: 6034229Abstract: Purified BMP-15-related proteins and processes for producing them are disclosed. DNA molecules encoding the BMP-15-related proteins are also disclosed. The proteins may be used in the treatment of bone and cartilage and/or other connective tissue defects and in wound healing and related tissue repair.Type: GrantFiled: December 2, 1997Date of Patent: March 7, 2000Assignees: Genetics Institute, Inc., Vanderbilt UniversityInventors: Anthony J. Celeste, Jennifer L. Dube, Karen M. Lyons, Brigid Hogan
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Patent number: 6027917Abstract: Purified BMP-17 and BMP-18 proteins and processes for producing them are disclosed. DNA molecules encoding the BMP-17 and BMP-18 proteins are also disclosed. The proteins may be used in the treatment of bone, cartilage, other connective tissue defects and disorders, including tendon, ligament and meniscus, in wound healing and related tissue repair, as well as for treatment of disorders and defects to tissues which include epidermis, nerve, muscle, including cardiac muscle, and other tissues and wounds, and organs such as liver, lung, epithelium, brain, spleen, cardiac, pancreas and kidney tissue. The proteins may also be useful for the induction of growth and/or differentiation of undifferentiated embryonic and stem cells.Type: GrantFiled: December 10, 1997Date of Patent: February 22, 2000Assignee: Genetics Institute, Inc.Inventors: Anthony J. Celeste, Beth L. Murray
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Patent number: 6027919Abstract: The present invention relates to a novel family of purified proteins, and compositions containing such proteins, which compositions are useful for the induction of tendon/ligament-like tissue formation, wound healing and ligament and other tissue repair. The present invention further relates to DNA molecules, vectors and host cells useful for production of such proteins.Type: GrantFiled: November 2, 1994Date of Patent: February 22, 2000Assignee: Genetics Institute, Inc.Inventors: Anthony J. Celeste, John M. Wozney, Vicki A. Rosen, Neil M. Wolfman, Gerald H. Thomsen, Douglas A. Melton
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Patent number: 6004937Abstract: Methods are provided for the modulation of the effects of GDF-8 and BMP-11, particularly on neural and muscular disorders administration of follistatin for treating neural, muscle, disorders which are characterized by an abnormality in the levels or activity of GDF-8 or BMP-11.Type: GrantFiled: March 9, 1998Date of Patent: December 21, 1999Assignee: Genetics Institute, Inc.Inventors: Clive R. Wood, Lori Jo Fitz
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Patent number: 5986056Abstract: Purified chordin proteins and processes for producing them are disclosed. DNA molecules encoding the chordin proteins are also disclosed. The proteins may be used in the treatment of bone, cartilage, other connective tissue defects and disorders, including tendon, ligament and meniscus, in wound healing and related tissue repair, as well for treatment of disorders and defects to tissue which include epidermis, nerve, muscle, including cardiac muscle, and othe tissues and wounds, and organs such as liver, brain, lung, cardiac, pancreas and kidney tissue. The proteins may also be useful for the induction inhibition of growth and/or differentiation of undifferentiated embryonic and stem cells. The proteins may be complexed with other proteins, particularly members of the transforming growth factor-beta superfamily of proteins.Type: GrantFiled: August 4, 1998Date of Patent: November 16, 1999Inventors: Edward R. LaVallie, Lisa A. Racie, Edward M. DeRobertis
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Patent number: 5965403Abstract: Purified BMP-16 proteins and processes for producing them are disclosed. DNA molecules encoding the BMP-16 proteins are also disclosed. The proteins may be used in the treatment of bone, cartilage, other connective tissue defects and disorders, including tendon, ligament and meniscus, in wound healing and related tissue repair, as well as for treatment of disorders and defects to tissues which include epidermis, nerve, muscle, including cardiac muscle, and other tissues and wounds, and organs such as liver, lung, cardiac, pancreas and kidney tissue. The proteins may also be useful for the induction of growth and/or differentiation of undifferentiated embryonic and stem cells.Type: GrantFiled: September 18, 1996Date of Patent: October 12, 1999Assignee: Genetics Institute, Inc.Inventors: Anthony J. Celeste, Beth L. Murray
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Patent number: 5958401Abstract: Provided by the present invention are methods of treating psoriasis using IL-11.Type: GrantFiled: December 10, 1997Date of Patent: September 28, 1999Assignee: Genetics Institute, Inc.Inventors: James Keith, Paul Schendel
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Patent number: 5948144Abstract: Apparatus and methods are disclosed for lyophilization of protein and/or pharmaceutical products, wherein said apparatus utilizes a dry vacuum pump for the direct removal of water vapor, rather than a cold trap condenser. A freeze dryer has a vacuum pump which is connected directly to a drying chamber without the use of a cold trap condenser. The exhaust of the vacuum pump is vented directly to atmosphere. Water vapor generated in the process is directly removed from the chamber by the vacuum pump. The apparatus permits lyophilization of pharmaceuticals e.g. antibiotics, vitamins products, vaccines, and biological protein solutions. The dryer operates on a batch basis or may be designed to perform continuous production.Type: GrantFiled: October 7, 1997Date of Patent: September 7, 1999Assignee: Genetics Institute, Inc.Inventor: Charles G. Cifuni
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Patent number: 5935852Abstract: DNA molecules are disclosed which encode mammalian Cerberus-Like Proteins.Type: GrantFiled: July 3, 1997Date of Patent: August 10, 1999Assignee: Genetics Institute, Inc.Inventors: Maximillian Follettie, Edward M. DeRobertis
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Patent number: 5932216Abstract: Purified Bone Morphogenetic Protein-10(BMP-10) proteins and processes for producing them are disclosed. DNA molecules encoding the BMP-10 proteins are also disclosed. The proteins may be used in the treatment of bone and cartilage defects and in wound healing and related tissue repair.Type: GrantFiled: September 10, 1997Date of Patent: August 3, 1999Inventors: Anthony J. Celeste, John M. Wozney
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Patent number: 5902785Abstract: Compositions of proteins with cartilaginous tissue inducing and maintenance activity are disclosed. The compositions are useful in the treatment of osteoarthritis, cartilage defects and in related tissue repair.Type: GrantFiled: May 7, 1996Date of Patent: May 11, 1999Assignee: Genetics Institute, Inc.Inventors: Gary Hattersley, Neil M. Wolfman, Elisabeth A. Morris, Vicki A. Rosen
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Patent number: 5888495Abstract: Lyophilized M-CSF formulations are presented that are free of high molecular weight species and stable to prolonged storage.Type: GrantFiled: August 18, 1992Date of Patent: March 30, 1999Assignee: Genetics Institute, Inc.Inventors: Jay A. Schrier, Donald F. Williams
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Patent number: 5883230Abstract: A noval fusion protein, IL3-X or X-IL3 wherein X is a hematopoietin fused to IL3.Type: GrantFiled: June 5, 1996Date of Patent: March 16, 1999Assignee: Genetics Institute, Inc.Inventor: Paul Schendel
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Patent number: 5861278Abstract: Purified HNF3.delta. proteins and processes for producing them are disclosed. DNA molecules encoding the HNF3.delta. proteins are also disclosed. The proteins may be used in regulating the transcription of one or more genes involved in the formation, differentiation, proliferation and maintenance of cells which form organ tissues, particularly pancreatic cells, as well as other tissue repair.Type: GrantFiled: November 1, 1996Date of Patent: January 19, 1999Assignee: Genetics Institute, Inc.Inventors: Gordon W. Wong, Kwok-Ming Yao