Abstract: The present invention relates to the development of a modified substrate capture immunoassay useful in the detection of a neutral metalloproteinase enzyme, type IV collagenase. Capture and immobilization of this enzyme can be achieved by coating the microtiter plate with gelatin, an alternative substrate for this enzyme. The captured enzyme can then be detected by affinity purified rabbit anti-type IV collagenase antibodies prepared against synthetic peptides from the amino terminus of the enzyme. Soluble type IV collagenase can readily be detected in samples known to contain this enzyme. Using purified enzyme this assay method can detect less than 50 ng of latent type IV collagenase. Using EDTA, an inhibitor of this metalloproteinase, gelatin binding can be shown to be independent of catalytic activity.

Abstract: The present invention relates to an oncoprotein specific for hepatocellular carcinomas and to a nucleotide sequence that codes for such a protein. The invention further relates to screening and diagnostic methodologies (and kits based thereon) that make use of the oncoprotein (or antibodies specific for same) and the nucleotide sequence.

Abstract: Continuous cell lines have been established from adrenocortical corcinomas. The cell lines are maintained in fully defined serum-free, steroid-free mediums. The cells of the invention, as exemplified by NCI-H295 cells, express all of the major pathways of steroid-ogenesis, including formation of corticosteroids, mineralocorticoids and androgens.

Abstract: The present invention relates to an oncoprotein specific for hepatocellular carcinomas and to a nucleotide sequence that codes for such a protein. The invention further relates to screening and diagnostic methodologies (and kits based thereon) that make use of the oncoprotein (or antibodies specific for same) and the nucleotide sequence.

Abstract: The invention concerns the isolation and sequencing of a new gene, arg, related to the abl proto-oncogene. The gene gets transcripted to two mRNA's; which in turn form two proteins. Antibodies, oligonucleotide probes and assays of detecting the arg gene, its mRNA and its protein products are also objects of the invention.

Abstract: The present invention relates to an oncoprotein specific for hepatocellular carcinomas and to a nucleotide sequence that codes for such a protein. The invention further relates to screening and diagnostic methodologies (and kits based thereon) that make use of the oncoprotein (or antibodies specific for same) and the nucleotide sequence.

Abstract: Biologically active proviral molecular clones of bovine immunodeficiency-like virus and cell lines infected with the same have been prepared. Various utilities of the clones are described.

Abstract: A complex of a cyclodextrin and an alkane, alkene, alkyne, aromatic compound, etc. can be prepared according to the method of the present invention. These complexes can be delivered to prokaryotic and eukaryotic cells, tissues, and organs in vitro and in vivo. In this manner, the toxic, genotoxic, and mitogenic effects of these compounds can be determined. Ternary complexes further including one or more biologically active molecules in addition to an alkane, etc. can be employed to determine the modulatory effects of such biologically active molecules on these alkanes, etc.

Abstract: A method of propagating human paramyxoviruses using a continuous cell line such as NCI-H292 (HuT-292), provides a suitable alternative to MK cells for the isolation and propagation of the human paramyxoviruses.

Abstract: The present invention relates to a unique physiologic model of chronic human immunodeficiency virus type-1 (HIV-1) infection. In particular, the present invention relates to a chronically infected promyelocyte cell line harboring a single integrated provirus. Unlike other models of chronic infection, the cell line of the present invention remain CD4.sup.+ under normal culture conditions during which <10% of the cells constitutively express HIV-1 proteins. However, when treated with tumor necrosis factor-alpha (TNF-.alpha.), the cell line dramatically increased (>35-fold) HIV-1 expression and rapidly down-modulated surface CD4, as >95% of the cells became HIV-1.sup.+. These results with the new OM-10.

Abstract: This application discloses probes for adenosine receptors which are functionalized congeners of the following compound: ##STR1## wherein R is --CH.sub.2 --C(O)--R' or ##STR2## These probes bind to A.sub.2 and A.sub.3 adenosine receptors and aid in quantifying and characterizing the receptors. The compounds may be labeled, for example with fluorescent compounds or radioactive compounds, or unlabeled.

Abstract: The present invention relates to a method of purifying macrophage stimulating protein (MSP) and to the highly purified MSP. The present invention further relates to antibodies to MSP. The highly purified MSP may be used for fighting pathogen infections.

Abstract: The present invention relates to the monoclonal antibody (termed D612) having selective reactivity for gastrointestinal carcinoma and methods for employing the same. A hybridoma producing such antibodies has been prepared.