Patents Represented by Attorney, Agent or Law Firm T K Ball
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Patent number: 6825006Abstract: Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding &dgr;-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the &agr; helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.Type: GrantFiled: July 22, 2002Date of Patent: November 30, 2004Assignee: Monsanto Technology LLCInventors: James A. Baum, Amy Jelen Gilmer, Anne-Marie Light Mettus
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Patent number: 6521442Abstract: Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.Type: GrantFiled: July 27, 2001Date of Patent: February 18, 2003Assignee: Monsanto Technology LLCInventors: Thomas Malvar, Amy Jelen Gilmer
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Patent number: 6372211Abstract: Compositions and methods for controlling insects by co-expressing an amino acid oxidase and a second enzyme that provides insecticidal activity when present in a mixture with the amino acid oxidase are disclosed. Also disclosed are DNA and protein sequences, and transformed microorganisms and plants useful for achieving such insect control.Type: GrantFiled: April 21, 1998Date of Patent: April 16, 2002Assignee: Monsanto Technolgy LLCInventors: Barbara G. Isaac, John T. Greenplate, John P. Purcell, Charles P. Romano
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Patent number: 6326351Abstract: Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae (Tribolium castaneum) and Japanese beetle larvae (Popillia japonica). Also disclosed are novel B. thuringiensis crystal toxin genes, designated cryET33 and cryET34, which encode respectively the coleopteran-toxic proteins, CryET33 (29-kDa) crystal protein, and CryET34 (14-kDa) crystal protein. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.Type: GrantFiled: July 21, 1999Date of Patent: December 4, 2001Assignee: Monsanto Technology LLCInventors: William P. Donovan, Judith C. Donovan, Annette C. Slaney
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Patent number: 6313378Abstract: Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding &dgr;-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the &agr; helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.Type: GrantFiled: June 21, 1999Date of Patent: November 6, 2001Assignee: Monsanto Technology LLCInventors: James A. Baum, Amy Jelen Gilmer, Anne-Marie Light Mettus
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Patent number: 6284949Abstract: A method for producing genetically transformed plants exhibiting toxicity to Coleopteran insects is disclosed. In another aspect, the present invention embraces chimeric plant genes, genetically transformed cells and differentiated plants which exhibit toxicity to Coleopteran insects. In yet another aspect, the present invention embraces bacterial cells and plant transformation vectors comprising a chimeric plant gene encoding a Coleopteran toxin protein of Bacillus thuringiensis.Type: GrantFiled: February 23, 1998Date of Patent: September 4, 2001Assignee: Monsanto CompanyInventors: David A. Fischhoff, Roy L. Fuchs, Paul B. Lavrik, Sylvia A. McPherson, Frederick J. Perlak
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Patent number: 6281016Abstract: Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and tansgenic plants expressing the modified endotoxin are also aspects of the invention.Type: GrantFiled: February 19, 1999Date of Patent: August 28, 2001Assignee: Monsanto CompanyInventors: Thomas Malvar, Amy Jelen Gilmer
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Patent number: 6221649Abstract: Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.Type: GrantFiled: March 2, 1999Date of Patent: April 24, 2001Assignee: Monsanto CompanyInventors: Thomas Malvar, Amy Jelen Gilmer