Patents Represented by Attorney, Agent or Law Firm The Law Office of Jonathan Alan Quine
-
Patent number: 6287862Abstract: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.Type: GrantFiled: July 26, 2000Date of Patent: September 11, 2001Assignee: Maxygen, Inc.Inventors: Stephen delCardayre, Matthew Tobin, Willem P. C. Stemmer, Jon E. Ness, Jeremy Minshull, Phillip Patten, Venkiteswaran Subramanian, Linda Castle, Claus M. Krebber, Steven H. Bass
-
Patent number: 6274337Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems.Type: GrantFiled: March 19, 1998Date of Patent: August 14, 2001Assignee: Caliper Technologies Corp.Inventors: J. Wallace Parce, Anne R. Kopf-Sill, Luc J. Bousse
-
Patent number: 6267858Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems.Type: GrantFiled: June 24, 1997Date of Patent: July 31, 2001Assignee: Caliper Technologies Corp.Inventors: J. Wallace Parce, Anne R. Kopf-Sill, Luc J. Bousse
-
Patent number: 6268184Abstract: The present invention relates to in situ hybridization methods for the identification of new chromosomal abnormalities associated with various diseases. In particular, it provides probes which are specific to a region of amplification in chromosome 20.Type: GrantFiled: April 24, 1998Date of Patent: July 31, 2001Assignee: The Regents of the University of CaliforniaInventors: Joe W. Gray, Colin Collins, Daniel Pinkel, Olli-Pekka Kallioniemi, Minna M. Tanner
-
Patent number: 6265716Abstract: A sample preparation method is disclosed for volatilization and mass spectrometric analysis of nonvolatile high molecular weight molecules. Photoabsorbing molecules having significant sublimation rates at room temperature under vacuum, and preferably containing hydroxy functionalities, are disclosed for use as matrices in matrix-assisted laser desorption/ionization mass spectrometry. The samples are typically cooled in the mass spectrometer to temperatures significantly below room temperature.Type: GrantFiled: February 29, 2000Date of Patent: July 24, 2001Assignee: GeneTrace Systems, Inc.Inventors: Joanna M. Hunter, Hua Lin, Christopher H. Becker
-
Patent number: 6261760Abstract: This invention provides a novel intracellular recycling free cholesterol pathway whose activity is required for cell division. The pathway provides the cholesterol needed for cell division prior to separation of daughter cells (mitosis). The new pathway offers several targets from pharmaceutical intervention, either via small molecules (such as sterol analogs) or by molecular engineering (preventing the cell from accumulating cholesterol by promoting free cholesterol efflux by transfection of caveolin cDNA).Type: GrantFiled: March 8, 1999Date of Patent: July 17, 2001Assignee: The Regents of The University of CaliforniaInventors: Christopher J. Fielding, Phoebe E. Fielding
-
Patent number: 6251674Abstract: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.Type: GrantFiled: February 7, 2000Date of Patent: June 26, 2001Assignee: Maxygen, Inc.Inventors: Matthew Tobin, William P. C. Stemmer, Jon E. Ness, Jeremy Minshull
-
Patent number: 6238538Abstract: Microfluidic devices are provided for the performance of chemical and biochemical analyses, syntheses and detection. The devices of the invention combine precise fluidic control systems with microfabricated polymeric substrates to provide accurate, low cost miniaturized analytical devices that have broad applications in the fields of chemistry, biochemistry, biotechnology, molecular biology and numerous other fields.Type: GrantFiled: April 6, 1999Date of Patent: May 29, 2001Assignee: Caliper Technologies, Corp.Inventors: John Wallace Parce, Michael R. Knapp, Calvin Y. H. Chow, Luc Bousse
-
Patent number: 6235471Abstract: Integrated systems, apparatus, software, and methods for performing biochemical analysis, including DNA sequencing, genomic screening, purification of nucleic acids and other biological components and drug screening are provided. Microfluidic devices, systems and methods for using these devices and systems for performing a wide variety of fluid operations are provided. The devices and systems of are used in performing fluid operations which require a large number of iterative, successive or parallel fluid manipulations, in a microscale, or sealed and readily automated format.Type: GrantFiled: April 3, 1998Date of Patent: May 22, 2001Assignee: Caliper Technologies Corp.Inventors: Michael Knapp, John Wallace Parce, Luc J. Bousse, Anne R. Kopf-Sill
-
Patent number: 6192475Abstract: A system and method for rewriting software into a protected form, called cloaked software, such that this cloaked form is protected from analysis or reverse engineering while at the same time the cloaked software is executable. Further, said cloaked software may be set up so that it requires a correct key or keys to be supplied, when it is to be run, for it to execute correctly. Cloaking modifies the basic operations within the software so that the logical connections or data flow among the program operations is no longer visible. In fact, cloaking makes the correct dataflow among operations dependent on a complex interrelated set of addressing operations within the cloaked program. These addressing operations are designed so that their analysis is equivalent to a computationally intractable NP-complete problem. This situation prevents reverse-engineering and unauthorized tampering.Type: GrantFiled: March 25, 1998Date of Patent: February 20, 2001Inventor: David R. Wallace
-
Patent number: 6162967Abstract: A method for introgressing soybean cyst nematode resistance into elite soybean germplasm is disclosed. The method involves using genetically mapped loci associated with soybean cyst nematode resistance for marker-assisted selection during introgression of soybean cyst nematode resistance into elite soybean germplasm. Also disclosed are a method for confirming selection for soybean cyst nematode resistance; quantitative trait loci associated with soybean cyst nematode resistance; and soybean lines bred to be resistant to soybean cyst nematode infestation. Also disclosed is a method for using the mapped markers for positional cloning of soybean cyst nematode resistance genes.Type: GrantFiled: June 13, 1997Date of Patent: December 19, 2000Assignee: Pioneer Hi-Bred International, Inc.Inventor: David M. Webb
-
Patent number: 6156181Abstract: Microfluidic devices are provided for the performance of chemical and biochemical analyses, syntheses and detection. The devices of the invention combine precise fluidic control systems with microfabricated polymeric substrates to provide accurate, low cost miniaturized analytical devices that have broad applications in the fields of chemistry, biochemistry, biotechnology, molecular biology and numerous other fields.Type: GrantFiled: October 26, 1998Date of Patent: December 5, 2000Assignee: Caliper Technologies, Corp.Inventors: John Wallace Parce, Michael R. Knapp, Calvin Y. H. Chow, Luc Bousse
-
Patent number: 6153073Abstract: The present invention generally provides microfluidic devices which incorporate improved channel and reservoir geometries, as well as methods of using these devices in the analysis, preparation, or other manipulation of fluid borne materials, to achieve higher throughputs of such materials through these devices, with lower cost, material and/or space requirements.Type: GrantFiled: August 11, 1999Date of Patent: November 28, 2000Assignee: Caliper Technologies Corp.Inventors: Robert S. Dubrow, Colin B. Kennedy, Luc J. Bousse
-
Patent number: 6090558Abstract: The present invention is related to the fields of genetic mapping and genetic identity detection, including forensic identification and paternity testing. This invention is more specifically directed to the use of mass spectrometry to detect length variation in DNA nucleotide sequence repeats (including variants of common alleles), such as microsatellites and short tandem repeats, and to DNA sequences provided as primers for the analysis of DNA tandem nucleotide repeat polymorphisms at specific loci on specific chromosomes.Type: GrantFiled: September 18, 1998Date of Patent: July 18, 2000Assignee: Genetrace Systems, Inc.Inventors: John M. Butler, Jia Li, Joseph A. Monforte, Christopher H. Becker
-
Patent number: 6087341Abstract: Skin cells are genetically altered to express a gene product encoded by an introduced polynucleotide. Specifically, the invention involves introduction of a nucleotide of interest into a skin cell by topical application of a polynucleotide that is substantially free of liposomes or charged lipids, where the skin can be either treated or untreated prior to application. Similarly, the invention involves introduction of a nucleotide of interest into a skin cell by topical application of a polynucleotide formulation comprising a liposome or charged lipid, wherein the skin is not treated by removal of hair prior to application. The method of the invention serves as a platform for delivering polynucleotides to skin cells for expression therein for any of a variety uses including, but not limited to genetic immunization.Type: GrantFiled: February 12, 1998Date of Patent: July 11, 2000Assignee: The Board of Trustees of the Leland Standford Junior UniversityInventors: Paul Khavari, Hongran Fan
-
Patent number: 6074725Abstract: Laminates having microfluidic structures disposed between sheets of the laminate are provided. The microfluidic structures are raised on a sheet of laminate, typically by printing the structure on the sheet. Printing methods include Serigraph, ink-jet, intaligo, offset printing and thermal laser printing.Type: GrantFiled: December 10, 1997Date of Patent: June 13, 2000Assignee: Caliper Technologies Corp.Inventor: Colin Kennedy
-
Patent number: 6068752Abstract: The present invention generally provides microfluidic devices which incorporate improved channel and reservoir geometries, as well as methods of using these devices in the analysis, preparation, or other manipulation of fluid borne materials, to achieve higher throughputs of such materials through these devices, with lower cost, material and/or space requirements.Type: GrantFiled: August 11, 1999Date of Patent: May 30, 2000Assignee: Caliper Technologies Corp.Inventors: Robert S. Dubrow, Colin B. Kennedy, Luc J. Bousse