Abstract: This invention discloses a method for chemically modifying a pulmonary surfactant protein or polypeptides with various fatty acids. These conjugates are useful in preparing formulations for the treatment of respiratory disease.

Abstract: The use of fiber to control infantile colic and diarrhea and adult constipation and diarrhea is well accepted. This invention is specifically directed to liquid nutritional formulas which contain hydrolyzed protein and/or amino acids and dietary fiber. This invention overcomes the problems of product physical stability and high viscosities through the use of soluble/non-fermentable fibers in combination with a blend of insoluble/non-fermentable and soluble/fermentable fibers. The invention is also directed to the use of physiologically effective levels of fibers in nutritional products that use hydrolyzed protein and/or free amino acids for the source of amino nitrogen to treat colic, diarrhea, short gut syndrome and other gastrointestinal maladies.

Abstract: The present invention relates generally to a method of producing plant proteins for use in nutritional products that have reduced levels of phytoestrogens, manganese or nucleotides. More specifically, this invention is directed to a method of using ion exchange technology to remove phytoestrogens, manganese or nucleotides from plant proteins. This invention is also directed to the plant protein product resulting from the inventive process and to nutritional products that use the plant protein product as a source of amino nitrogen.

Abstract: A process of treating infection by a pathogenic microorganism in a subject in need of such treatment including the step of administering to the subject an effective antimicrobial amount of diacetyltartaric acid esters of mono- and diglycerides is provided. Pharmaceutical compositions containing diacetyltartaric acid esters of mono- and diglycerides as an active antimicrobial agent are also provided.

Abstract: Methods and compositions are provided for a rapid quantitative nucleic acid hybridization assay for detecting a DNA or RNA sequence in a biological sample. The method comprises solution hybridization of a target nucleic acid sequence with a detectably-labeled probe, separation of the target-nucleic acid probe from the unhybridized probe by gel exclusion or affinity chromatography and detection of the amount of labeled probe as an indication of the target nucleic acid present in the biological sample.

Abstract: There is disclosed structured lipid containing either a gamma-linolenic acid or a dihomogamma-linolenic acid residue, together with an n-3 fatty acid residue and a medium chain fatty acid residue on the same glycerol backbone. This structured lipid is particularly well adapted to the treatment of disease or stress states. The gamma-linolenic or dihomogamma-linolenic acid residues modify the prostanoid synthesis pathway, reducing the level of series "2" prostanoids and elevating the levels of series "1" and "3" prostanoids. The n-3 fatty acid residue enhances the level of series "1" prostanoids as well as increases the production of series "3" prostanoids. The medium chain fatty acid residues enhances the absorption of the structured lipid. There is also disclosed enteral and parenteral diets as well as nutritional supplements containing the structured lipids of the invention.

Abstract: A nutritional formulation containing bactericidal amounts of diacetyltartaric acid esters of mono- and diglycerides is provided. A process of inhibiting bacterial infections including the step of feeding the nutritional composition to a subject is also provided.

Abstract: An enteral nutritional product for a person having ulcerative colitis contains in combination (a) an oil blend which contains eicosapentaenoic acid (20:5n3) and/or docosahexaenoic acid (22:6n3), and (b) a source of indigestible carbohydrate which is metabolized to short chain fatty acids by microorganisms present in the human colon. Preferably the nutritional product also contains one or more nutrients which act as antioxidants.

Abstract: A DNA probe assay is described using neutrally charged probe strands.

Abstract: A method of treating pneumonia or other respiratory disease caused by respiratory syncytial virus, adenovirus, parainfluenza virus, or influenza virus by orally administering a liquid, preferably an infant formula, containing a virus neutralizing antibody.

Abstract: This invention relates to a palatable elemental nutritional formula that is nutritionally complete for humans with specialized dietary needs. The nutritional of the present invention uses specific free amino acids to provide the source of amino nitrogen (protein equivalents) and a special blend of fats that provides 38 to 50% of the total Calories in a pleasant tasting formula. The nutritionally complete formula of this invention is useful for children having multiple protein allergies, short gut syndrome, sick gut, diarrhea and the like. More specifically, the nutritional product, in accordance with this invention, utilizes L-asparagine monohydrochloride and L-glutamine in place of L-aspartic acid and L-glutamic acid, respectively. In addition, the source of fat comprises soy, fractionated coconut oil (medium chain triglycerides), high oleic safflower oil and esterified glycerol emulsifiers.

Abstract: Process methods are described for combining compounds of mineral nutrients into chemically stable powder mixtures that notably preserve chromium in a soluble form conducive to both absorption and bioavailability. The methods include the compounds of minerals in preferred chemical forms, procedures for treatment of certain compounds to maintain solubility, appropriate sequences for combination of the compounds and other premix ingredients, and blending to provide an adequately homogeneous mixture. The methods provide premixes of minerals that support bioavailable chromium and that exhibit good stability, solubility, odor and color for addition to nutritional products.

Abstract: A process of treating a bacterial infection in a subject in need of such treatment including the step of administering to the subject an effective bactericidal amount of diacetyltartaric acid esters of mono- and diglycerides is provided. Pharmaceutical compositions containing diacetyltartaric acid esters of mono- and diglycerides as an active bactericidal agent are also provided.

Abstract: A novel method for the prevention of infectious diarrhea or diarrhea caused by antibiotic therapy is disclosed. The method comprises the steps of 1) mixing a powder comprising viable cultures of the probiotic organisms Lactobacillus reuteri, Lactobacillus acidophilus and Bifidobacieriurn infantis with a liquid; and 2) enterally administering the mixture to a mammal or a human. In a preferred embodiment at least 10.sup.6 CFU (colony forming units) of each probiotic organism is consumed per day. The invention also relates to pills or capsules containing the probiotic system (Lactobacillus reuteri, Lactobacillus acidophilus and Bifidobacterium infantis) in a freeze dried or lyophilized form. The invention also relates to a novel powdered nutritional formula for the prevention of diarrhea that comprises protein, fat, carbohydrates and the microorganisms Lactobacillus reuteri, Lactobacillus acidophilis and Bifidobacterium infantis.

Abstract: A diagnostic method for assessing how a hypercholesterolemic person's serum cholesterol level will respond to a low total fat and saturated fat diet. The method involves defining a target cholesterol level, measuring a baseline level, feeding for a term of 3-25 days a NCEP Step I diet of which at least 50% of the caloric intake is provided by a fortified, low-fat nutritional product, and comparing the post term cholesterol level to the defined goal to see if the target is met. If the target is met, the person is predicted to respond to dietary intervention. If the target is not met, the person is deemed a non-responder and is considered for drug therapy. A fortified, low-fat nutritional product supplies in about 1000 kcal (i) 100% of the adult RDI for vitamins and minerals; and (ii) total fat, saturated fat and cholesterol levels that are at or below the levels specified for a Step II diet.

Abstract: Fatty acid esters such as those containing arachidonic acid (AA) and docosahexaenoic acid (DHA) derived from lipid mixtures are prepared with reduced levels of sterols and phosphorus. A preferred embodiment of the invention comprises extracting lipids from egg with methanol; separating lipids including sterols from insoluble egg components; submitting the methanolic solution of lipids to transesterification and subsequent neutralization to convert the lipids to methyl esters of said free fatty acids together with sterols; separating the said sterols and esters from an aqueous phase including phosphorus compounds formed in the transesterification; subjecting the said fatty acid esters and sterols to distillation to separate sterols from the fatty acid esters; and subjecting the said esters to transesterification in the presence of glycerol to produce triglycerides of said esters thereof including that of AA and DHA wherein the resulting triglycerides contain reduced quantities of sterols and phosphorus.

Abstract: The invention relates to multiplex ligase chain reaction (LCR). Two or more purative target sequences are selected. For each one, a set of four probes is used simultaneously to amplify the putative sequence if it is present in the sample. Preferably, all the amplicons are labeled with a common label/hapten and, for each different target, with a unique label/hapten. The invention also relates to an immunochromatographic strip device and method employing a diagonal array of capture spots.

Abstract: A nutritional formulation containing an amount of diacetyltartaric acid esters of mono- and diglycerides effective to inhibit the infectious activity of pathogenic microorganisms is provided. A process of inhibiting the infectious activity of pathogenic microorganisms including the step of feeding the nutritional composition to a subject is also provided.

Abstract: Two oligonucleotides are provided which function as amplifying primers when used in tandem in the polymerase chain reaction with HPV DNA. The primers are of the formulae:IU: 5'-TIIN.sub.1 IN.sub.1 IIN.sub.2 TAAAACGAAAGT-3'andIWDO: 5'-N.sub.1 TCN.sub.1 N.sub.3 AIGCCCAN.sub.2 TGIACCAT-3'wherein N.sub.1 is A or G, N.sub.2 is C or T and N.sub.3 is A or T.

Abstract: The present invention provides an adenosine kinase protein having an identified structural formula. The identified protein or similar adenosine kinase proteins having residues as claimed and recited herein may be isolated and purified from natural sources or produced by recombinant DNA technology.