Abstract: The present invention is directed to a simple and efficient process for the recovery of a biologically active glycoprotein from a biological fluid containing it.In a preferred embodiment, it comprises:a) contacting a filtered culture fluid with a dihydroxyboronyl bearing chromatographic supportb) eluting with a first eluting buffer and contacting this eluate directly with an anion exchange matrix bearing quaternary ammonium functional groupsc) eluting with a second eluting buffer and collecting the product therefrom.