Abstract: A self-contained, leak-proof sampling device and kit employing said device for collecting chemical and biological samples from various surfaces. The invention provides a sampling device and kit that may be employed to easily collect chemical and biological samples, safely transport the collected samples, safely dispense collected samples for analysis and provide optimum sample recovery. The sampling device is in the form of a leak proof container that comprises a lid and base. The lid contains a sterile absorbent collection means integrated into and positioned on the inside surface of the lid. The base contains a means to facilitate sample recovery from the absorbent collection means via compression and/or scraping of the absorbent collections means. Methods for employing the present invention are described herein.

Abstract: An improved crew mask for protection of a user against chemical and biological hazards is provided. The novel crew mask includes the following features: a facepiece that includes a hybrid face seal with both intern and flat seals, and at least one transparent lens positioned at the level of the eyes of the person; at least one inlet hose for delivery of filtered air operably connected to the facepiece; at least one exhaust one-way exhaust port for venting air from the crew mask; and a compact filter unit with a reduced airflow resistance relative to currently employed filter units.

Abstract: A biodegradation process for the organophosphonate product of Sarin (O-isopropyl methylphosphonofluoridate) hydrolysis, i.e., isopropylmethylphosphonate (IMPA). This process provides a feasible biodegradation demilitarization alternative to Sarin incineration. Public opposition of nerve agent incineration is widespread, and alternative methods are sought to help the U.S. Army meet the 2007 demilitarization deadline imposed by the Chemical Weapons Convention. This process uses a two-step approach to IMPA biodegradation. In the first step, a concentrated IMPA solution is used as the sole nutritional carbon and phosphorus source for microbial cultures. The second step involves diluting the culture and adding an inexpensive carbon source to encourage bacterial phosphate assimilation. The biodegradation typically involves a consortium of microorganisms comprising Methylobacterium radiotolerans GB21, Agrobacterium tumefaciens GB2GA, Klebsiella oxytoca GB2CS, GB272, Aureobacterium sp.

Abstract: A continuous monitoring method and system wherein a porous substrate or film is used. The air from the environment is drawn through a region of the porous substrate by a simple air pump and the substances in the air are deposited or chemically adsorbed onto the surface of the substrate. The region of the substrate where the environmental air is drawn through is continuously monitored by an optical or spectrometric method. The substrate is in the form of a tape supplied by a feed reel in a reel-to-reel cartridge and taken up by a take-up reel as found in a film cartridge or a magnetic tape cartridge. The cartridge can be replaceable. A variety of materials may be employed as the substrate with an adequate surface area to effect accumulation of solid, liquid, aerosol, or gas phase compounds.

Abstract: The invention provides particle detectors and detection methods. A particle detector includes a spherical/ellipsoid shell comprising spherical and ellipsoid mirrors that define a focal point within an interior of the shell, a pair of opposing tubes passing through the spherical/ellipsoid shell and directed at the focal point for directing particles to the focal point, and a light source directed at the focal point for directing light at the particles to generate fluorescence from the particles at or near the focal point.

Abstract: A twist lock mount for quick mounting and positioning a device to a support includes a mount receiving member attachable to or integral with the support and a mounting member attachable to or integral with a device to be mounted. The receiving member provides a channel covered by flanges that further define a narrower channel or slot. The mounting member is configured to slide into the channel when it is in one rotational orientation and to lock securely into place when it is in another. A rotation in the opposite direction will unlock the device so that the mount can be slid out of the channel quickly and easily. The twist lock mount simply and effectively prevents movement in the longitudinal, transverse and axial directions and is well suited for use in harsh, high vibration environments.

Abstract: A containment and test system for extremely toxic substances includes a fume hood, a containment compartment such as glove bag or box, positioned within the fume hood, a source of a non-reactive gas to provide a stable environment within the containment compartment, an inline moisture trap coupled to the source of non-reactive gas to dry the non-reactive gas, an ion neutralizer coupled to the source of non-reactive gas to reduce static charges within the containment compartment, a staging area for preparing test samples, a balance positioned within the containment compartment, a stanchion positioned within the fume hood for holding a test animal, and decontaminating apparatus for safely disposing of any toxic substance residue.

Abstract: Methods are described for detecting chemical warfare agents that contain organophosphorus compounds. Compositions containing either (1) a sufficient amount of phosphonate ester hydrolase with an alkali agent or (2) a sufficient amount of phosphonate ester hydrolase, a sufficient amount of organophosphorus hydrolase and a sufficient amount of a organophosphorus acid anhydrolase, may be utilized to test for organophosphorus compound-containing chemical warfare agents. These enzymes will react with such chemical warfare agents to produce degradation products, specifically phosphonate esters.

Abstract: The invention provides purified and isolated DNA fragments from Bacillus anthracis chromosomal DNA, primer sets and probes derived therefrom, as well as kits and detection methods for B. anthracis. The methods of the invention provide for specific detection of anthrax over closely related strains of Bacillus, as well as accurate detection of low numbers of B. anthracis in an environmental sample containing large amounts of non-specific DNA. The invention is applicable to food, health care, and military applications.

Abstract: An explosive destruction system and method for safely destroying explosively configured chemical munitions. The system comprises a sealable, gas-tight explosive containment vessel, a fragment suppression system positioned in said vessel, and shaped charge means for accessing the interior of the munition when the munition is placed within the vessel and fragment suppression system. Also provided is a means for treatment and neutralization of the munition's chemical fills, and means for heating and agitating the contents of the vessel. The system is portable, rapidly deployable and provides the capability of explosively destroying and detoxifying chemical munitions within a gas-tight enclosure so that there is no venting of toxic or hazardous chemicals during detonation.

Abstract: The present invention concerns a method for detecting the presence of a ribosome inactivating protein in a sample. The method comprises contacting a sample suspected of containing a ribosome inactivating protein with a 2?-O-methylated oligonucleotide substrate having a GAxGA tetraloop wherein “Ax” is a deoxyribonucleoside comprising a fluorescent adenine derivative or analog base capable of emitting a fluorescence when released from the nucleoside, “Ax”, and detecting the presence of the fluorescent adenine derivative or analog base released from the tetraloop as an indication of the presence of the ribosome inactivating protein in the sample. The present invention is also directed to an assay kit and a reagent useful for carrying out the steps of the above method.

Abstract: The invention provides reactive sorbents and methods of making and using the same in order to decontaminate surfaces contaminated with toxic agents, such as chemical warfare agents and/or industrial toxins. The reactive sorbents are of two general types, one of which comprises dehydroxylated aluminum oxide and the other comprises porous carbon impregnated with a reactive solution, so that both sorbents take up and then detoxify toxic agents.

Abstract: The invention provides purified and isolated DNA fragments from Bacillus anthracis chromosomal DNA, primer sets and probes derived therefrom, as well as kits and detection methods for B. anthracis. The methods of the invention provide for specific detection of anthrax over closely related strains of Bacillus, as well as accurate detection of low numbers of B. anthracis in an environmental sample containing large amounts of non-specific DNA. The invention is applicable to food, health care, and military applications.

Abstract: The invention provides purified and isolated DNA fragments from Bacillus anthracis chromosomal DNA, primer sets and probes derived therefrom, as well as kits and detection methods for B. anthracis. The methods of the invention provide for specific detection of anthrax over closely related strains of Bacillus, as well as accurate detection of low numbers of B. anthracis in an environmental sample containing large amounts of non-specific DNA. The invention is applicable to food, health care, and military applications.

Abstract: The present invention relates to methods and assays for detecting bacteriophage MS2 in a sample.

Abstract: A method of enzymatic degradation of hydrolyzed nerve agents GB, GD, GF, VX and O-isobutyl S-(diethyl-aminoethyl) methylphosphonothioate into biodegradable methylphosphonate products using enzymes from bacteria that can use for growth as the sole phosphorus source, low concentrations of ethyl-isopropyl-, and pinacolyl methylphosphonates (EMPn, IMPn, and PMPn-alkali treatment products of VX, GB, and GD, respectively).

Abstract: A chemical/biological protection helmet is provided with a filter system. In order to provide for improved balance control and visibility, the filter system is positioned in the rear of the helmet. By rotating the visors, provided in the front of the helmet, the filter system can be actuated as the helmet is sealed.

Abstract: The invention provides purified and isolated DNA fragments from Bacillus anthracis chromosomal DNA, primer sets and probes derived therefrom, as well as kits and detection methods for B. anthracis. The methods of the invention provide for specific detection of anthrax over closely related strains of Bacillus, as well as accurate detection of low numbers of B. anthracis in an environmental sample containing large amounts of non-specific DNA. The invention is applicable to food, health care, and military applications.

Abstract: The invention provides purified and isolated DNA fragments from Bacillus anthracis chromosomal DNA, primer sets and probes derived therefrom, as well as kits and detection methods for B. anthracis. The methods of the invention provide for specific detection of anthrax over closely related strains of Bacillus, as well as accurate detection of low numbers of B. anthracis in an environmental sample containing large amounts of non-specific DNA. The invention is applicable to food, health care, and military applications.

Abstract: A particle aerosol belt has a double-seal construction. A first zip-lock container contains the dry aerosol particles, such as brass metallic flake. The zip-lock containers are then placed in an elongate tubing. The tubing is sealed between the zip-lock containers to form a plurality of cells in which the zip-lock containers are disposed. A method of forming the particle aerosol belt includes introducing the brass metallic flake or other material, in the form of a slurry into the zip-lock containers, and drying the slurry, prior to sealing the zip-lock containers.