Abstract: A spectrally resolvable set of rhodamine dyes are provided for use in the chain termination method of nucleic acid sequencing. A different rhodamine dye from the group consisting of tetramethylrhodamine, rhodamine X, rhodamine 6G, and rhodamine 110 is attached to the base of each of the dideoxynucleotides used in the sequencing method by way of an alkynylamino linker. Preferably, the labeled dideoxynucleotides are incorporated into the growing DNA chains by Taq DNA polymerase.
Type:
Grant
Filed:
September 29, 1989
Date of Patent:
November 22, 1994
Assignee:
Applied Biosystems, Inc.
Inventors:
B. John Bergot, Vergine Chakerian, Charles R. Connell, J. Scott Eadie, Steven Fung, N. Davis Hershey, Linda G. Lee, Steven M. Menchen, Sam L. Woo