Abstract: A variable-orifice fluid restrictor for use with a supercritical extractor or chromatograph includes an inlet line for fluid at a pressure above its critical pressure, an extended tubular probe having an inner and an outer surface and a proximal and a distal end. The proximal end of the probe is disposed toward the inlet line. The distal end of the probe includes an adjustable orifice means adapted for metering the fluid and having first and second orifice members and an adjusting stem having first and second ends. The adjustable orifice means is adjacent to the outer surface of the probe and the orifice means is adjustable with the adjusting stem. The end of the adjusting stem is located at the distal end of the probe and is adapted for moving the first orifice member with respect to the second orifice member to control the adjustable orifice for varying the restriction of fluid passing through the adjustable orifice.

Abstract: An apparatus for supercritical fluid extraction incorporates a removable extraction cartridge which in operation has insignificant pressure difference between its inside and outside walls. Because of the low pressure difference, the extraction cartridge need not have the strength to withstand significant pressure and can be made out of molded plastic for disposable use as well as stainless steel and/or machined plastic for reusability. The extraction cartridge can be removed and opened for sample access without the use of tools. The outside of the cartridge can be purged after it is installed in a heated high pressure vessel to remove contamination from its exterior. In one embodiment, the extractor includes a fraction collector for extractants, an automatic sample changer and an automatic cartridge transfer mechanism which provide completely automated extractions.

Abstract: Daunomycinone is a known precursor of the well known antibiotic and antineoplastic, doxorubicin. Daunomycinone may be synthesized by preparing mono ketal of a 1,4-naphthoquinone as the precursor of the CD rings of daunomycinone followed by preparing a precursor for ring A with C.sub.6 and C.sub.11 attached and the stereochemistry at C.sub.7 and C.sub.9 established and coupling it to CD and then completing ring B.

Abstract: To collect analyte in a supercritical fluid extraction process, the extractant flows to a collection container under pressure. In one embodiment, the collection container contains collection solvent through which the extractant flows to partition analyte. The extractant adds to the liquid and when the collection solvent-extractant mixture rises to a heating zone, the extractant vaporizes selecively since its vapor pressure is higher than the vapor pressure of the collection solvent. When its pressure exceeds a preset valve pressure it is vented so that extractant continually leaves the collection container. After the extraction is complete, the analyte is concentrated in the collection solvent. In another embodiment, the collection vessel includes a solid material on which the analyte collects. It is removed under pressure by a solvent that is concentrated under pressure.

Abstract: To select a designated bottle in a sampler, the distributor is calibrated as to position and the outlet of the distributor arm is moved over the desired bottle by counting changes in incremental angular movement of the distributor arm until it is over the bottle. The position of the distributor arm is recorded in memory. To calibrate the distributor, the arm is rotated against a stop member in a first direction, then in a second direction and the overrun of in excess of 360 degrees of the motion of the distributor arm is determined and corrected. A series of random numbers is generated for depositing of samples, the distributor is moved at designated times over the bottles in a random order to deposit a sample and the ordering of bottles is recorded. The rollers of a peristaltic pump are supported to prevent said rollers from compressing said tube more than the thickness of the walls of the tube. A functional module for a bubbler or pH probe or ultrasonic level detector may be connected to the housing.

Abstract: To degauss cassettes of magnetic tape, a magnetic field is applied to the magnetic material while the magnetic material moves with respect to the field in a single air gap of a flux density of at least 1,000 gauss and at an angle between 20 degrees and 70 degrees from the horizontal of the magnetic tape for a time priod of at least one second and the field is alternated at a frequency of at least ten hertz. The magnetic material may be rotated while it is in the field.

Abstract: To sequence DNA automatically, DNA marked with far infrared, near infrared, or infrared fluorescent dyes are electrophoresed in a plurality of channels through a gel electrophoresis slab or capillary tubes wherein the DNA samples are resolved in accordance with the size of DNA fragments in the gel electrophoresis slab or capillary tubes into fluorescently marked DNA bands. The separated samples are scanned photoelectrically with a laser diode and a sensor, wherein the laser scans with scanning light at a wavelength within the absorbance spectrum of said fluorescently marked DNA samples and light is sensed at the emission wavelength of the marked DNA.

Abstract: To determine the average velocity of a fluid stream, an ultrasonic signal is transmitted into the fluid and reflected ultrasonic signal received. The signals are mixed with a frequency of the transmitted ultrasonic signals. A Fourier transform is performed on the signals, the largest coefficient used to normalize the signal and certain of the weighted signals are averaged.

Abstract: To alter feedstock material, the material is exposed to laser radiation applied at a selected angle of incidence, intensity and wavelength related to the refractive index of the feedstock material. Fine uniform particles may be formed through vapor explosion and/or plasma formation and used by this method to coat surfaces, such as with paint or adhesive or to supply uniform small particles to a heat engine. Moreover, moving materials such as a column of liquid may be subjected to high internal pressure and temperature for creating physical and chemical changes.

Abstract: To sequence DNA automatically, fluorescently marked DNA are electrophoresed in a plurality of channels through a gel electrophoresis slab; wherein the DNA samples are resolved in accordance with the size of DNA fragments in the gel electrophoresis slab into fluorescently marked DNA bands. The separated samples are scanned photoelectrically with a laser and a sensor, wherein the laser scans with scanning light at a scanning light frequency within the absorbance spectrum of said fluorescently marked DNA samples and light is sensed at the emission frequency of the marked DNA. The light is modulated from said laser at a predetermined modulation frequency and fluorescent light emitted by said DNA bands at said modulation frequency is detected, whereby background noise from the medium through which the light is transmitted is discriminated against.

Abstract: A fluid sampling apparatus includes a universal base, a plurality of inner container carriers, a sequencer means, a means for holding an inner container carrier within the base, and an insulated cooler. The universal base is sufficiently large to receive any one of a plurality of different configurations of container holders or composite containers and includes locating members to properly position the different composite containers or container carriers. The container carriers include a sleeve or wall formed to have on its inner surface a plurality of indentations, each shaped and sized to receive a different one of the containers in fixed relationship to permit easy distribution of samples in the proper container.

Abstract: To couple a driving shaft of a machine to a driven shaft, a coupling assembly is mounted to the driving and driven shafts and includes a flexible coupling element. The flexible coupling element includes a plurality of first fasteners adapted to be connected to the driving shaft and a plurality of second fasteners adapted to be connected to the driven shaft. A plurality of fibers connects different ones of the first fasteners to different ones of the second fasteners so that the first fasteners applies force to the second fasteners to transmit torque. The filaments or fibers are looped around the fasteners and the coupling element has a thickness in the range of 1 percent to 10 percent of its diameter.

Abstract: To sequence DNA, DNA samples marked with flourscent infrared dye are applied at a plurality of locations for electrophoresing in a plurality of channels through a gel electrophoresis slab. The channels are scanned with a laser and a sensor, that include a microscope focused on the gel slab. The focal point and slab are adjusted with respect to each other so that the focal point of the microscope remains on the gel slab during a scan. The data from the scan is directly used to amplitude modulate density readings on a display, and the scan is displayed in a horizontal sweep of a cathode ray tube, whereby said cathode ray tube provides intensity displays of bands representing DNA. Different sizes of glass gel sandwiches may be mounted to the same console for different sequencing tasks.

Abstract: To determine the total organic carbon content of a flowing liquid in a continuous process, water and oxidizing agents enter lower ports in a multistage column and flow upwardly through residence chambers as they are mixed. Spaced apart along the upper path of the liquid, there are helical reaction grooves relatively shallow and containing at least one helical groove to cause rapid motion of the liquid. An ultraviolet lamp extends downwardly through the center of the column so that the outer confining surface of the helical grooves is the outer surface of the ultraviolet lamp, causing the liquid to move rapidly in intimate contact with the ultraviolet lamp to receive intense radiation. The stages are separate and interchangeable so that selected sizes may interconnect. The residence chambers may have different sizes to permit different lengths of residence time and thus permit tailoring of the reaction to the expected organic carbon content and possible side reactions with other impurities in the water.

Abstract: To control the speed of a lawn mower, the speed of the lawn mower is changed in a forward direction along a longitudinal plane, resulting in a tendency to turn because of different speed characteristics of the right and left wheel drives. The speed is corrected by lateral movement of a control lever to increase the speed of one wheel motor and decrease the speed of the other wheel motor. When turning, if the lawn mower is not aligned along the longitudinal axis after the turn, the deviation is indicated in the position of the control lever to the right or left. This can be corrected by momentarily applying a motor speed change to one of said right and left hydraulic motors while observing the control lever until the control lever is centered in the longitudinal plane, whereby the speed of the lawn mower will be aligned along the longitudinal plane.

Abstract: To erect concrete structures, precast concrete beams are formed, columns having void spaces therein are erected and angle irons are temporarily mounted to said columns at beam level wherein said beams may be temporarily supported. At least one beam is positioned orthogonal to a column near the void in the column supported by the angle iron and cast-in-place concrete fills the void between the ends of said beams and said columns. In another embodiment, the columns are solid and the beams have openings of such a size as to fit around the columns so that they can be lowered to the proper beam height about the column and fastened, with the other ends of the beam being joined to adjacent beams.

Abstract: To form a binder from naturally occurring products without extraction or purification steps, the binder utilizes proteins from the germ or endosperm of cereal grasses or from the seed of buckwheat, oil seed plants, Amaranthus or leguminous plants or from leaves. The protein has a thermoplastic microstructure with linear polymers of moleculer weight of at least 2,000 linked with peptide linkages of at least 50 in number. The naturally occurring product is ground or milled without further processing and thus includes carbohydrates, particularly as cellulose, and possibly fats, yeast or materials yielding ash upon burning, with the carbohydrates being at least 5 percent of the proteins by weight. The binder may include some initiators or catalysts to polymerize fats or other initiators or catalysts to polymerize proteins.

Abstract: To prepare a biodegradable plastic, biodegradable materials such as starches and a non-biodegradable polymer such as a polystyrene, polyurethane, polyethylene, polypropylene, or polycarbonate are treated: (1) under heat, pressure and reagents to break the polymers; and (2) by adding to them an oxidizing agent. This treatment forms and/or makes available reactive groups for bonding: (1) on the biodegradable material groups such as aldehyde or hydroxyl groups in the case of the carbohydrates and amine groups in the case of proteins and certain other compounds such as urea; and (2) on the non-biodegradable plastic groups such as aldehydes, methyl, propyl, ethyl, benzyl or hyroxyl groups.