Abstract: Methods and compositions are provided for in vitro expansion of growth factor dependent cells. Expansion is effected through the use of growth factor conjugates that include a growth factor such as a steel factor and a polysaccharidase substrate binding region. The conjugates are immobilized by binding of the substrate binding region to a substrate of the polysaccharidase in a growth chamber for the cells.
Type:
Grant
Filed:
January 16, 1996
Date of Patent:
February 23, 1999
Assignee:
University of British Columbia
Inventors:
Douglas G. Kilburn, Keith R. Humphries, James G. Doheny, Eric Jervis, Judie Alimonti