Abstract: The synthesis of a new affinity medium, the p-aminophenyl-.gamma.-ester of 7-methylguanosine-5'-triphosphate coupled to Sepharose, for use in affinity chromatography is claimed. An improved method in terms of speed and purity for the isolation of messenger ribonucleic acid cap-binding protein is claimed. The synthesis of the p-aminophenyl-.gamma.-ester of 7-methylguanosine-5'-triphosphate is described.
Abstract: An improved method of electroblotting macromolecules from a chromatographic gel is claimed. The method comprises a design for wire electrode arrays that are capable of generating either highly uniform or controlled predetermined gradient electric fields. A gradient electric field is particularly suited for the quantitative electroblotting of proteins with a wide range of molecular weights.
Type:
Grant
Filed:
November 7, 1983
Date of Patent:
September 17, 1985
Assignee:
Yale University
Inventors:
Fred E. Davis, III, Johathan M. Gershoni
Abstract: Topical administration of one of the polyoxygenated Labdanes of which forskolin is an example to the eyes produces lower intraocular pressure. The topical application to the eye produces no significant changes in blood pressure or pulse rate. The polyoxygenated Labdanes are effective agents for the treatment of glaucoma.
Abstract: This invention makes use of the action of ocean waves, converting the water power to electrical energy. This is accomplished by increasing the size of the wave and converting the wave action to hydraulic power, using a turbine and a generator. With this method energy can be stored in tanks (under pressure) and used when needed.