Abstract: This application discloses a method of inhibiting degradation of the antidepressant bupropion hydrochloride in a solid pharmaceutical formulation, so that the pharmaceutical formulation will maintain at least 80% of its initial bupropion potency after one year.

Abstract: A method for purifying and recovering biologically active somatotropin monomers from refold solution following the solubilization and naturation of refractile bodies of host cells produced by recombinant DNA methodology. The purification process is based on the discovery that somatotropin monomers and somatotropin oligomers having overlapping isoelectric points may nevertheless be separated by selective precipitation over a narrow pH range. Host cell residues including proteins, pyrogens and other impurities present in the refold solution are effectively removed in the process. The purified somatotropin monomers recovered from solution after removing precipitated solids are suitable for parenteral application to target animals without further purification.

Abstract: Solid dosage forms of bioactive materials for parenteral administration such as porcine or bovine somatotropin pellets intended for subcutaneous implantation to improve food production of farm animals are coated with polyvinyl alcohol to extend the release characteristics of the material. The polyvinyl alcohol preferably has a molecular weight of from about 20,000 to 100,000, a degree of hydrolysis of at least about 98%, and is applied from an aqueous solution by spray coating to form a continuous uniform covering having a weight of from about 3 to 25 ug/mm.sup.2.

Abstract: A method for preparing polypeptides in bacteria with an alanine rather than a methionine at the N-terminus. The DNA sequence expressed has an alanine codon immediately following from at least one methionine codon, and allows for the expression of polypeptides having the amino acid sequence essentially the same as such naturally occurring eucaryotic protein as various bovine and porcine somatotropin species. In another important embodiment, the invention provides a class of compositions comprising certain valine-containing somatropin species which increase milk production in lactating mammals to a significantly greater extent than otherwise-identical leucine somatotropin species.

Abstract: Variant somatotropins are described in which the asparagine located in the 95-101 region are replaced by glutamine which somatotropins exhibits enhanced stability.

Abstract: The growth of milk-producing mammary parenchyma in a mammal can be significantly enhanced by administering to the mammal by intramammary infusion a substance having a mitogenic effect on mammary epithelial cells in said mammal. Good growth enhancement can be achieved using very small quantities of such substances, and in relatively short periods of time.

Abstract: The growth of milk-producing mammary parenchyma in a mammal can be significantly enhanced by administering to the mammal by intramammary infusion a substance having a mitogenic effect on mammary epithelial cells in said mammal. Good growth enhancement can be achieved using very small quantities of such substances, and in relatively short periods of time.

Abstract: A therapeutic ointment having wet adherent properties and particularly well suited for delivery of medicaments to mucosal surfaces includes a base material comprising from about 3 to 15% by weight of a water soluble salt of a copolymer of methyl vinyl ether and maleic acid or anhydride in a polyethylene glycol ointment. Formulations of the base material with triamcinolone acetonide and lidocaine are particularly efficacious in the treatment of recurrent aphthous ulcers.

Abstract: A method for the solubilization and naturation of somatotropin from refractile bodies produced by r-DNA technology wherein the refractile bodies are dissolved in an aqueous solution comprising a chaotropic agent such as urea or guanidine hydrochloride and a soluble organic alcohol such as isopropanol or benzyl alcohol. The solubilized protein is exposed to mild oxidation for a time sufficient to allow the protein to form disulfide bonds and refold to its native conformation. The presence of the alcohol suppresses the formation of somatotropin dimers and aggregates and results in higher yields of the desirable monomeric form of the protein.

Abstract: Solid dosage forms of bioactive materials for parenteral administration such as porcine or bovine somatotropin pellets intended for subcutaneous implantation to improve food production of farm animals are coated with polyvinyl alcohol to extend the release characteristics of the material. The polyvinyl alcohol preferably has a molecular weight of from about 20,000 to 100,000, a degree of hydrolysis of at least about 98%, and is applied from an aqueous solution by spray coating to form a continuous uniform covering 3 to 25 .mu.m PVA/mm.sup.2.

Abstract: Variant forms of mammalian somatotropins, particularly bovine and porcine somatotropin, in which the asparagine located between positions 95-101 has been replaced by glutamine exhibit enhanced stability at high pH and at elevated temperatures while retaining bioactivity equivalent to the corresponding unmutated somatotropins, and are useful in the treatment of animals to enhance growth and/or milk production.

Abstract: A method for the solubilization and naturation of somatotropin from refractile bodies produced by r-DNA technology wherein the refractile bodies are dissolved in an aqueous solution comprising a chaotropic agent such as urea or guanidine hydrochloride and a soluble surfactant such as sodium dodecylsulfate. The solubilized protein is exposed to mild oxidation for a time sufficient to allow the protein to form disulfide bonds and refold to its native conformation. The presence of the surfactant suppresses the formation of somatotropin dimers and aggregates and results in higher yields of the desirable monomeric form of the protein.

Abstract: A process is provided for producing nitrophenetole where nitrochlorobenzene is reacted in a cosolvent of a monohydric alcohol and N-alkylpyrrolidone with aqueous alkali metal hydroxide.

Abstract: Prolonged parenteral release of a bioactive polypeptide at desirably effective levels can be achieved using novel compositions in which the polypeptide is present in a biocompatible oil in an unusually high proportion such as at least about 10% by weight. Also disclosed are certain metal-associated somatotropins that are useful for prolonged parenteral release of such somatotropins.

Abstract: Liquid loaded pads useful as wound and burn dressings are prepared from pellicles of microbially-produced cellulose obtained, for example, by culturing Acetobacter xylinum. A pellicle having a thickness from about 0.1 to 15 millimeters or greater is processed to replace the culture medium with water or other physiologically compatible liquid. The liquid-loaded pellicle is sterilized prior to its use as a dressing or in other medical applications.

Abstract: A sterilization process wherein articles contacted with hydrogen peroxide and containing trace amounts of residual hydrogen peroxide are subjected to plasma treatment to generate an active species from the residual hydrogen peroxide which effects sterilization of the article and concurrently removes the residual hydrogen peroxide by converting it into non-toxic decomposition products.

Abstract: A light weight entangled nonwoven fabric formed by fluid rearrangement/entangling of an oriented web of fibers comprising at least 75% polyolefin staple fibers and displaying excellent machine direction and cross direction strength.

Abstract: A container comprising a tubular body members of rectangular cross-section and a hinged lid member having a skirt formed by shallow depending walls adapted to telescope closely over the walls of the body member when the lid is in the closed position, is provided with latching means and a projection on the outside of each side wall of the body member adjacent the open end thereof having an interference fit with the inside surface of the side wall of the lid member, whereby compressive forces are exerted by the lid member on the side walls of the body member as the lid is closed, thereby causing the front wall of the body member to flex slightly to assure the positive engagement of the latching means.

Abstract: Ultraviolet light absorbing compositions comprising 2-(2'-hydroxy-5'-acryloyloxyalkoxyphenyl(-2H-benzotrizoles, and copolymers thereof with one or more other monomers copolyermizable therewith, particularly acrylic monomers, are useful in the manufacture of ocular devices, particularly intraocular lenses and contact lenses.