Abstract: Plasmid vectors are provided that carry complementary DNA (cDNA) clones cng for polypeptides exhibiting murine interleukin-2 activity. One of these polypeptides is 168 amino acids in length, including a potential leader sequence of about 19 amino acids. The cDNA is derived from messenger RNA isolated from a mouse T-cell line after activation with concanavalin A. The cDNA was cloned by incorporation into a plasmid vector, which is then transformed into E. coli. The plasmid vector also contained DNA segments from the SV40 virus, permitting expression of the cDNA after transfection into a mammalian host cell, such as monkey COS-7 cells.
Type:
Grant
Filed:
December 17, 1986
Date of Patent:
January 17, 1989
Assignee:
DNAX Research Institute of Molecular and Cellular Biology, Inc.
Inventors:
Frank D. Lee, Takashi Yokota, Ken-ichi Arai