Abstract: This invention relates to a test element and a method for determining the ratio of glycated to non-glycated haemoglobin in a sample, for example, in a blood sample that is provided as a fresh sample, a dried sample or a haemolysed sample. The test element can include different zones for receiving the sample, serving as a depot for reagents such as non-immobilized signal-generating molecules or haemolysis reagents, separating reagents from the sample, and detecting heamoglobin. The zones are provided on a porous matrix and in fluid contact with each other. The method uses the test element and determines the ratio of glycated to non-glycated haemoglobin in a sample, by contacting the sample with an excess of signal generating molecules to bind to the glycated haemoglobin. The non-bound signal generating molecules are separated from the sample, and the amount or concentration of haemoglobin and/or glycated haemoglobin bound signal generating molecules are determined.