Abstract: The present invention describes terminal phosphate blocked nucleoside polyphosphates that are stable at high temperature and their use in nucleic acid amplification and analysis. Current invention further describes charge modified terminal phosphate blocked nucleoside polyphosphates for improved incorporation and direct loading of nucleic acid sequencing reactions onto separating media.
Type:
Grant
Filed:
August 29, 2003
Date of Patent:
August 14, 2007
Assignee:
GE Healthcare Bio-Sciences Corp.
Inventors:
Anup Sood, Shiv Kumar, Carl Fuller, John Nelson