Abstract: Methods and compositions to minimize barcode exchange during the preparation of barcoded next-generation sequencing libraries prepared from a single cell. The methods utilize oligonucleotides containing a 3?-terminated blocking group or sequences that prevent amplification or extension.
Type:
Grant
Filed:
August 2, 2019
Date of Patent:
December 10, 2024
Assignee:
10X GEMOMICS, INC.
Inventors:
Jason Bell, Josephine Lee, Corey Nemec, Francesca Meschi