Abstract: Systems and methods are provided performing autonomous tuning of a stringed instrument. Images of the stringed instrument are acquired by at least two cameras and processed to identify the location of a peg. A robotic peg actuator is then engaged to actuate rotation of a peg in order to rotate the peg to a tuned angular orientation, guided by feedback generated by processing acoustic signals recorded by an acoustic transducer while a string associated with the peg is agitated. In some example implementations, the cameras and peg actuator are rigidly mounted to a frame that is configured to support the stringed instrument during peg imaging and actuation. A robotic string actuator may be provided to facilitate vibration of the string during the tuning process. Multiple robotic peg actuators may be included for per-peg rotational actuation and/or a given peg actuator may be robotically translatable to actuate more than one peg.