Abstract: This invention provides methods and kits for enriching and/or detecting a nucleic acid with at least one variant nucleotide from a nucleic acid population in a sample. Methods employ the use of enriching primers and bridge-probes for enriching and detecting target nucleic acids. Extension of the enriching primer permits amplification of the target nucleic acid having the variant nucleotide.
Abstract: The present invention provides methods and kits for detecting the presence or absence or amount of a target nucleic acid or at least one variant nucleotide in one or more target nucleic acids contained in a sample using appropriate primers. The invention is based on systems whereby there is extension-dependent degradation of the primers in the presence of the target, which degradation leads to a detectable signal.
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