Abstract: An applicator includes a main body, a storage chamber disposed in the main body, an application body disposed at an end of the main body to allow application of the liquid stored in the storage chamber. A partition for partitioning between the storage chamber side and the application body side, includes a flow passage to supply the liquid in the storage chamber to the application body. An axially movable air communication pipe having an air port open to the storage chamber, which abuts on the partition to close the flow passage, communicates the storage chamber with the atmosphere while having the flow passage closed. A spring member for urging the air communication pipe to abut on the partition, and separating the air communication pipe from the partition through vibration of the main body allows guiding of the liquid in the storage chamber.

Abstract: An applicator includes a partition wall for partitioning between a storage chamber and a reservoir chamber, an application body that allows application of a liquid stored in the storage chamber, a relay member that transfers the liquid stored in the storage chamber passing through the partition wall toward the application body, a gas-liquid exchanging section formed at the partition wall for air-liquid exchanging with the liquid stored in the storage chamber, an occluding body that is disposed in the reservoir chamber, and in contact with the relay member for occluding the liquid, and a movement prevention section disposed at the relay member and/or the occluding body for preventing movement of the liquid held in the occluding body to the relay member.

Abstract: Disclosed herein are methods and compositions (e.g., oligonucleotide primers) for isothermal amplification and detection of M. pneumoniae nucleic acids in a sample. In some embodiments, the methods include contacting a sample with a set of LAMP primers specific for a M. pneumoniae CARDS toxin-encoding nucleic acid under conditions sufficient to produce an M. pneumoniae nucleic acid amplification product and detecting the resulting M. pneumoniae amplification product. Kits including sets of LAMP primers for detection of M. pneumoniae CARDS toxin nucleic acids are also provided herein.