Abstract: The present invention concerns a sterile closed loop device for medical-surgical use intended for the collection, the transportation and the processing of prostheses and/or biological samples and the like, having at least one collection compartment provided with an hermetically closable main opening, for the introduction of said prosthesis and/or biological samples and the like to be analyzed, so as to be contained in it, at least one containment chamber, in which a solution is contained for the chemical detachment of bacteria adhering to animate or inanimate surfaces, such as prostheses and the like, and/or bacterial biofilm, said containment chamber being selectively communicating with said collection compartment, so that said solution can be conveyed from said containment chamber to said collection compartment, for the chemical separation of bacteria to said prosthesis and/or biological samples and the like to be analyzed, that can be inserted in said collection compartment, and extraction means of said so