Abstract: A dielectric coated plasmonic photoemitter is provided. An aspect of the present photonic apparatus includes a conductive photoemitter including a dielectric material coating or layered on a metallic core. The dielectric material being configured to enhance a local optical field strength and current density of the photoemitter as compared to a bare photoemitter without the dielectric layer. The dielectric layered photoemitter being tunable to transmit photoemissions from corners thereof with different photonic characteristics depending on a laser wavelength pulse received.

Abstract: The present invention relates to a cell culture medium for preparing liver, gastric, pancreatic, colon or intestinal adult stem cell isolated from adult tissue, as well as for maintaining such stem cell in the undifferentiated state. The cell culture medium comprises a base medium; an ABL and SRC dual kinase inhibitor/an ABL kinase inhibitor and a SRC kinase inhibitor; a mitogenic factor; a WNT signalling pathway activator; a stimulator for NAD+ and NADP+ generation; and a cAMP/PKA pathway activator. In a particular embodiment, the ABL and SRC dual kinase inhibitor is Dasatinib; the mitogenic factor is EGF; the WNT signalling pathway activator is R-Spondin 1; the stimulator for NAD+ and NADP+ generation is nicotinamide; and the cAMP/PKA pathway activator is cholera endotoxin.

Abstract: There are provided new heterobifunctional agents designed to mediated formation of protein-protein dimers and promote ubiquitination of a protein of the dimer. Also provided are methods of synthesizing the agents, pharmaceutical formulations including the agents, and methods of using the agents to treat, ameliorate or cure diseases characterized by protein over-expression or malfunction.

Abstract: Among other things, a method for normalizing a sample is provided. In some embodiments, the method comprises: (a) reacting a sample with a limiting amount of a single-turnover sequence-specific endonuclease that recognizes a target sequence, thereby cleaving a portion of the nucleic acid molecules that comprise the target sequence and producing a normalized amount of a first cleavage product; and (b) isolating, transcribing or selectively amplifying the normalized amount of the first cleavage product. In this method, because a limiting amount of the endonuclease is used, the normalized amount of the first cleavage product is determined by the limiting amount of the first single-turnover sequence-specific endonuclease used in step (a).

Abstract: The present invention relates to an unbiased and simultaneous amplification method for preparing a double-stranded DNA library from a sample of more than one type of nucleic acid. In particular, the present invention provides an unbiased and simultaneous amplification method for preparing a double-stranded DNA library from more than one type of nucleic acid in substantially low amount comparative to non-nucleic acid molecules in the sample within a relatively shorter turnaround time and substantially without any purification step between amplifications and between cDNA preparation and amplification as compared to conventional methods of preparing DNA library.

Abstract: A system and method are presented for sharing an audio-visual stream from a server system with a plurality of participant computer systems and for the automated generation of portions. Each participant computer system provides audio and visual data which is converted into the audio-visual stream by the server system. The server system then shares the audio-visual stream with the participant computer systems. During the audio-visual stream, a participant computer can request a portion at a time T. The server will automatically generate a portion audio-visual file using audio and visual data from the stream extending from before time T to after time T. After creation of the portion, the participant computer can modify the timing of the portion and can modify the visual presentation of the portion.

Abstract: We describe the use of an exosome for the preparation of a pharmaceutical composition to promote or enhance would healing or hair growth, or both, in an individual. The exosome may be derived from a stem cell such as a mesenchymal stem cell (MSC).

Abstract: A system and method are presented for deep linking within a social media message. This is accomplished by allowing a link in the message to be wrapped by a social media server. The wrapped link is then opened in a browser embedded in a social media app, resulting in the display of a web page in the embedded browser that requests a user email address. When this email address is provided back to a server, an email message to that address is sent. The email address can directly include the deep link. When this deep link is opened in an email app, this link can directly perform a deep link into a second app since the user is now outside the embedded browser of the social media app. Alternatively, a web page accessed from the email message opens in a non-embedded browser, which then presents the deep link.

Abstract: A system and method are presented for content control and monitoring in a multi-party video stream using a specialized user interface (UI). Particular participants are identified as luminaries and allowed to specify off-limit topics. Other participants are presented UIs that require agreement to avoid off-limit topics. All participants are immediately able to report problem behavior during the stream through the provided UI. Reports made through this interface immediately result in quarantining compensation and streaming content including reports relating to the off-limit topics. Administrative UIs allow for quicker understanding of the reporting issues, quicker review of the potentially offending stream, and quicker resolution of the report.

Abstract: A system and method are presented for sharing an audio-visual stream from a server system with a plurality of participant computer systems and for the automated generation of portions. Each participant computer system provides audio and visual data which is converted into the audio-visual stream by the server system. The server system then shares the audio-visual stream with the participant computer systems. During the audio-visual stream, a participant computer can request a portion at a time T. The server will automatically generate a portion audio-visual file using audio and visual data from the stream extending from before time T to after time T. After creation of the portion, the participant computer can modify the timing of the portion and can modify the visual presentation of the portion.

Abstract: A system and method are presented for the creation and management of live audio-visual streams between multiple parties. An organizing computer system requests a system server to establish a stream with a limited, preset number of slots. Other computer systems request to join the stream. Once the present number of slots is filled, a queue is established for waiting computer systems. Participating computer systems can be limited in their participation to a present time period, after which they are removed from the streams. Removal of one computer system from the stream opens a slot for another, which is filed from the queue of waiting computer systems. The queue can be sorted through a variety of means, including FIFO and auction.

Abstract: A system and method are presented for content control and monitoring in a multi-party video stream using a specialized user interface (UI). Particular participants are identified as luminaries and allowed to specify off-limit topics. Other participants are presented UIs that require agreement to avoid off-limit topics. All participants are immediately able to report problem behavior during the stream through the provided UI. Reports made through this interface immediately result in quarantining compensation and streaming content including reports relating to the off-limit topics. Administrative UIs allow for quicker understanding of the reporting issues, quicker review of the potentially offending stream, and quicker resolution of the report.

Abstract: A system and method are presented for the creation and management of live audio-visual streams between multiple parties. An organizing computer system requests a system server to establish a stream with a limited, preset number of slots. Other computer systems request to join the stream. Once the present number of slots is filled, a queue is established for waiting computer systems. Participating computer systems can be limited in their participation to a present time period, after which they are removed from the streams. Removal of one computer system from the stream opens a slot for another, which is filed from the queue of waiting computer systems. The queue can be sorted through a variety of means, including FIFO and auction.

Abstract: We describe a particle secreted by a mesenchymal stem cell and comprising at least one biological property of a mesenchymal stem cell. The biological property may comprise a biological activity of a mesenchymal stem cell conditioned medium (MSC-CM) such as cardioprotection or reduction of infarct size. The particle may comprise a vesicle or an exosome.

Abstract: We describe the use of exosome such as mesenchymal stem cell exosomes in a method of promoting, restoring or enhancing homeostasis in an individual suffering from graft versus host disease (GVHD) or epidermolysis bullosa (EB). The homeostasis may comprise immune homeostasis such as maintenance of an immune response. The method may comprise administering a therapeutically effective amount of exosome to the individual.

Abstract: We describe a method of monitoring the state of a cell, tissue, organ or organism. The method comprises establishing, for a sample of micro-particles from the cell, tissue, organ or organism, a ratio. The ratio is of a selected polypeptide in microparticles which comprise GM1 gangliosides, preferably which bind to Cholera Toxin B (CTB) (“GM1 ganglioside microparticle polypeptide”) to the selected polypeptide in microparticles which comprise exposed phos—photidylserine, preferably which bind to Annexin V (“Annexin V microparticle polypeptide”). The GM1 ganglioside microparticle polypeptide to Annexin V microparticle polypeptide ratio so established may be indicative of the state of the cell, tissue, organ or organism.

Abstract: A method for constructing a sequencing library based on a single-stranded DNA molecule is provided comprising: (1) forming a poly(C)n tail at a 3?-terminus of the single-stranded DNA molecule, to obtain a single-stranded DNA molecule with the poly(C)n tail with n representing a number of base C, and n being an integer ranging from 5 to 30; (2) obtaining a double-stranded DNA molecule by using an extension primer based on the single-stranded DNA molecule with the poly(C)n tail, with the extension primer comprising a H(G)m unit at a 3?-terminus thereof, H being base A, base T or base C, m being a number of base G, and m being an integer ranging from 5 to 15; and (3) ligating an adapter to one terminus of the double-stranded DNA molecule remote from the H(G)m unit, and amplifying the resulting ligation product to obtain an amplification product forming the sequencing library.

Abstract: Described herein are therapeutic compositions from cultured mesenchymal stem cells, and methods relating to their preparation and use.

Abstract: The present disclosure provides multipotent endoderm spheroid progenitor cells, liver organoids, intestinal organoids, and pancreatic spheroids, and methods for producing same from stem and progenitor cells.

Abstract: We describe a method of detecting pre-eclampsia in a cell, tissue, organ or organism, the method comprising detecting a modulated level of expression, activity or amount of a pre-eclampsia biomarker polypeptide selected from the group consisting of PlGF, FLT1, BNP, ANP, CD9, PAI-1, TGF ?, PCT, SI 00b, TIMP1, CD 105 and IL6 in or of a microparticle type (selected from a CTB binding microparticle and an Annexin V binding microparticle) from the cell, tissue, organ or organism, as compared to level of expression, activity or amount of the pre-eclampsia biomarker polypeptide in the same microparticle type in a cell, tissue, organ or organism not sufferin from pre-eclampsia.