Abstract: A test strip for determining blood glucose concentration includes a test strip and a photometer. The test strip comprises a substrate formed with an aperture, a polymeric membrane adhered to the aperture and in registration with the aperture, having a quantity of reagent on its bottom surface of the membrane. The polymeric membrane is adhered to the bottom of the substrate by double sided tape. The placement of the reagent on the bottom surface of the membrane allows filtration of the red blood cells from plasma before the glucose in the blood reaches the reagent and carries with it oxygen to drive the glucose-reagent reaction to completion. The photometer includes an infrared LED for detecting glucose components in the reacted test strip and simple, one-button operation.