Abstract: A connecting polypeptide has SEQ ID NO:73. A proinsulin polypeptide includes a mature insulin A-chain, a mature insulin B-chain, and a connecting peptide comprising SEQ ID NO: 73 linking the mature A-chain and the mature B-chain, wherein the connecting peptide is not a native human proinsulin C-peptide. The proinsulin polypeptides according to the invention can be made in high titers and in high purity.

Abstract: The present invention is an inducible coexpression system, capable of controlled induction of expression of each gene product.

Abstract: The present invention provides host cells having an oxidizing cytoplasm, which are capable of growing to high cell densities and producing recombinant proteins in soluble form. Also provided are methods for growing such host cells to high densities, methods for optionally modulating the growth rates of the host cells, and methods for inducing the host cells to produce the desired gene product in soluble form. Further aspects of the invention relate to host cell preparation and storage methods, which utilize the advantages of producing gene products in host cell cytoplasm to provide methods for long-term storage and/or transport of expressed gene products that are retained in a stable and soluble form within the host cells.

Abstract: The present invention provides host cells for use in an inducible coexpression system that is capable of controlled induction of expression of each gene product.

Abstract: The present invention provides expression vectors for use in an inducible coexpression system, capable of controlled induction of expression of each gene product.

Abstract: The present invention is an inducible coexpression system, capable of controlled induction of expression of each gene product.

Abstract: The present invention provides expression vectors for use in an inducible coexpression system, capable of controlled induction of expression of each gene product.