Abstract: Provided are defined culture media for culturing pluripotent stem cells in a suspension culture devoid of substrate adherence, the defined culture media comprising an effective amount of a protease inhibitor; a GSK3? inhibitor and at least one agent selected from the group consisting of a protease inhibitor and a WNT3A polypeptide; a WNT3A polypeptide and a stabilizing agent thereof with the proviso that said stabilizing agent is not a lipid vesicle; and/or a GSK3? inhibitor, with the proviso that the medium is devoid of an ERK1/2 inhibitor. Also provided are cell cultures and methods of suing same.

Abstract: Provided are methods of deriving a mammalian livestock pluripotent stem cells line, by (a) ex-vivo culturing a mammalian livestock embryo of at least 7 days post-fertilization for a culturing period of at least 4 days and no more than until 21 days post-fertilization so at to obtain an embryo comprising epiblast cell and/or late stage pluripotent stem cell; (b) isolating from the embryo the epiblast cell and/or the late stage pluripotent stem cell, and (c) culturing the epiblast cell and/or the late-stage pluripotent stem cell under conditions suitable for expansion of undifferentiated mammalian livestock pluripotent stem cells to thereby obtain a population of mammalian livestock pluripotent stem cells. Also provided are isolated mammalian livestock pluripotent stem cells, and cells differentiated therefrom.

Abstract: Culture media capable of promoting differentiation of pluripotent stem cells (PSCs) into mesenchymal stem cells (MSCs) or supporting expansion of MSCs is provided. Methods of differentiation of PSCs into MSCs are provided. Methods of expanding MSCs without differentiation are also provided.

Abstract: Provided are defined culture media for culturing pluripotent stem cells in a suspension culture devoid of substrate adherence, the defined culture media comprising an effective amount of a protease inhibitor; a GSK3? inhibitor and at least one agent selected from the group consisting of a protease inhibitor and a WNT3A polypeptide; a WNT3A polypeptide and a stabilizing agent thereof with the proviso that said stabilizing agent is not a lipid vesicle; and/or a GSK3? inhibitor, with the proviso that the medium is devoid of an ERK1/2 inhibitor. Also provided are cell cultures and methods of suing same.