Patents Assigned to ACEA BioSciences
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Publication number: 20130123136Abstract: A method of performing an assay of a response of two or more cell types to a test compound, including: providing a device for monitoring cell-substrate impedance, adding at least two different cell types to the device; adding at least one test compound to form at least two test compound wells; providing at least two control wells; monitoring impedance of the at least two test compound wells and of the at least two control wells at three or more time points after adding the at least one test compound; and analyzing measured impedance from the at least two test compound wells and from the at least two control wells at the three or more time points to obtain information on the response of the different cell types to the at least one test compound.Type: ApplicationFiled: December 19, 2012Publication date: May 16, 2013Applicant: ACEA BIOSCIENCES, INCInventor: ACEA BIOSCIENCES, INC
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Publication number: 20130100443Abstract: A prism including a substrate faceted to provide a plurality of flat surfaces, wherein at least two of the plurality of surfaces, each including a filter coating, form at least two filter surfaces, wherein each filter surface selectively permits passage of a predetermined wavelength and reflects remaining wavelengths along an optical path towards another of the plurality of surfaces, optionally another filter surface, wherein an angle of incidence of each of the plurality of surfaces along the optical path is equal or nearly equal. An apparatus incorporating the prism and its use for splitting a light spectrum into a plurality of wavelengths or wavelength ranges.Type: ApplicationFiled: October 19, 2012Publication date: April 25, 2013Applicant: ACEA BIOSCIENCES, INCInventor: ACEA BIOSCIENCES, INC
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Patent number: 8420363Abstract: A device for detecting cells and/or molecules on an electrode surface is disclosed. The device detects cells and/or molecules through measurement of impedance changes resulting from the cells and/or molecules. A disclosed embodiment of the device includes a substrate having two opposing ends along a longitudinal axis. A plurality of electrode arrays are positioned on the substrate. Each electrode array includes at least two electrodes, and each electrode is separated from at least one adjacent electrode in the electrode array by an expanse of non-conductive material. The electrode has a width at its widest point of more than about 1.5 and less than about 10 times the width of the expanse of non-conductive material. The device also includes electrically conductive traces extending substantially longitudinally to one of the two opposing ends of the substrate without intersecting another trace. Each trace is in electrical communication with at least one of the electrode arrays.Type: GrantFiled: December 1, 2008Date of Patent: April 16, 2013Assignee: Acea Biosciences, Inc.Inventors: Xiaobo Wang, Xiao Xu
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Patent number: 8344742Abstract: The invention provides methods of investigating a mechanism of action of a compound, which includes providing a device for monitoring cell-substrate impedance; attaching the device to an impedance analyzer; adding cells to two or more wells; adding a test compound to at least one of the wells and providing at least one control well; monitoring impedance of the wells to obtain a series of impedance measurements; plotting impedance measurements to obtain impedance curves and comparing the impedance curves to determine a time frame at which the test compound has a significant effect on cell growth or behavior. Determining the time frame provides information on changes in cell status in response to the test compound, including cell attachment or adhesion status, cell growth or proliferation status, the number of viable or dead cells, cytoskeletal organization or structure, and the number of cells going through apoptosis or necrosis.Type: GrantFiled: January 21, 2011Date of Patent: January 1, 2013Assignee: ACEA Biosciences, Inc.Inventors: Yama A. Abassi, Xiaobo Wang, Xiao Xu
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Patent number: 8334316Abstract: The present invention provides substituted di-, tri-, tetra- and penta-sulfide compounds and compositions, and methods of using the same for the treatment and/or prevention of a cell proliferative disorder. The present invention also provides methods for preparing trisulfide compounds and compositions.Type: GrantFiled: February 9, 2009Date of Patent: December 18, 2012Assignee: ACEA Biosciences, Inc.Inventors: Xiao Xu, Haoyun An, Xiaobo Wang
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Patent number: 8263375Abstract: The present application includes systems and methods for identifying a compound capable of interacting with a G-Protein Coupled Receptor (GPCR) or Receptor Tyrosine Kinase (RTK) including providing a device capable of measuring cell-substrate impedance operably connected to an impedance analyzer, adding test cells expressing a GPCR or a RTK to wells of the device, measuring first impedances of the wells and optionally determining first cell indices from the first impedances, adding a compound to at least one well containing test cells to form at least one compound well and adding a vehicle control to at least another well containing test cells to form at least one control well, measuring second impedances of the compound well and the control well and optionally determining second cell indices from the second impedances, determining the change in the impedance or cell index for the compound well and the one control well, comparing the change in impedance or cell index between the compound well and the controlType: GrantFiled: August 4, 2005Date of Patent: September 11, 2012Assignee: ACEA BiosciencesInventors: Yama A. Abassi, Naichen Yu, Josephine Atienza, Xiao Xu, Xiabo Wang
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Publication number: 20120225424Abstract: The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.Type: ApplicationFiled: May 17, 2012Publication date: September 6, 2012Applicant: ACEA BIOSCIENCES, INC.Inventors: Yama A. Abassi, Xiaobo Wang, Josephine Atienza, Xiao Xu, Junquan Xu
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Patent number: 8252822Abstract: Novel compounds having a fused bicyclic heteroaromatic ring system substituted with a heteroaryl five-membered ring are disclosed. The compounds inhibit growth of a variety of types of cancer cells, and are thus useful for treating cancer. Efficacy of these compounds is demonstrated with a system for monitoring cell growth/migration, which shows they are potent inhibitors of growth and/or migration of cancer cells. In addition, compounds of the invention were shown to stop growth of tumors in vivo, and to reduce the size of tumors in vivo. Compositions comprising these compounds, and methods to use these compounds and compositions for treatment of cancers, are disclosed.Type: GrantFiled: July 17, 2008Date of Patent: August 28, 2012Assignee: Acea Biosciences, Inc.Inventors: Haoyun An, Biao Xi, Yama Abassi, Xiaobo Wang, Xiao Xu
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Patent number: 8206903Abstract: The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.Type: GrantFiled: November 23, 2005Date of Patent: June 26, 2012Assignee: Acea BiosciencesInventors: Xiaobo Wang, Yama A. Abassi, Josephine Atienza, Xiao Xu, Junquan Xu
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Publication number: 20110300569Abstract: A method of determining one or more beating parameters for use in cardiomyocyte beating analysis including: providing a cell analysis device including wells, each well including a sensor capable of monitoring beating of cardiomyoctes in millisecond time resolution; adding cardiomyocytes to the wells; monitoring the beating of the cardiomyocytes in millisecond time resolution to obtain a plurality of beating measurements; and calculating one or more beating parameters from the plurality of beating measurements.Type: ApplicationFiled: May 17, 2011Publication date: December 8, 2011Applicant: ACEA BIOSCIENCES, INC.Inventors: Nan Li, Xiaobo Wang, Yama A. Abassi, Biao Xi, Wen Fu Zhang, Xiao Xu
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Patent number: 8041515Abstract: The present invention provides methods of multi-dimensional profiling of biologically active agents and determining their effects on biological systems. The methods of the present invention include real-time impedance monitoring of cellular responses to biologically active agents and categorization of cellular kinetic profiles into mechanism specific cellular response profile groups. The grouping of similar cellular response profiles allows the correlation between agent and mechanism, thus allowing for the identification of potential therapeutic applications of agents or further study of cellular responses or mechanisms.Type: GrantFiled: September 20, 2007Date of Patent: October 18, 2011Assignee: Acea Biosciences, Inc.Inventors: Xiaobo Wang, Yama A. Abassi, Wen fu Zhang, Xiao Xu
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Patent number: 8026080Abstract: The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. In some preferred aspects, the assays are designed to investigate the affects of compounds on cells, such as cytotoxicity assays.Type: GrantFiled: March 15, 2007Date of Patent: September 27, 2011Assignee: Acea Biosciences, Inc.Inventors: Xiaobo Wang, Yama A. Abassi, Xiao Xu, Jiangbo Gan
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Publication number: 20110117542Abstract: The invention provides methods of investigating a mechanism of action of a compound, which includes providing a device for monitoring cell-substrate impedance; attaching the device to an impedance analyzer; adding cells to two or more wells; adding a test compound to at least one of the wells and providing at least one control well; monitoring impedance of the wells to obtain a series of impedance measurements; plotting impedance measurements to obtain impedance curves and comparing the impedance curves to determine a time frame at which the test compound has a significant effect on cell growth or behavior. Determining the time frame provides information on changes in cell status in response to the test compound, including cell attachment or adhesion status, cell growth or proliferation status, the number of viable or dead cells, cytoskeletal organization or structure, and the number of cells going through apoptosis or necrosis.Type: ApplicationFiled: January 21, 2011Publication date: May 19, 2011Applicant: ACEA BIOSCIENCES, INCInventors: Yama Abassi, Xiaobo Wang, Xiao Xu
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Patent number: 7876108Abstract: The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. The methods can be used to test the effects of compounds on cells, such as in cytotoxicity assays.Type: GrantFiled: June 8, 2009Date of Patent: January 25, 2011Assignee: ACEA Biosciences, Inc.Inventors: Yama A. Abassi, Xiaobo Wang, Xiao Xu
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Patent number: 7851657Abstract: The invention provides a synthetic and manufacturing process for the preparation of the anticancer drug, fluorapacin, bis(4-fluorobenzyl)trisulfide, and related trisulfide derivatives on large scale. Also provided are processes for the purification and isolation of fluorapacin having high purity and improved stability.Type: GrantFiled: March 27, 2009Date of Patent: December 14, 2010Assignee: Acea Biosciences, Inc.Inventors: Haoyun An, Xiaopeng Mo
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Publication number: 20100191016Abstract: The invention provides a synthetic and manufacturing process for the preparation of the anticancer drug, fluorapacin, bis(4-fluorobenzyl)trisulfide, and related trisulfide derivatives on large scale. Also provided are processes for the purification and isolation of fluorapacin having high purity and improved stability.Type: ApplicationFiled: March 27, 2009Publication date: July 29, 2010Applicant: ACEA BIOSCIENCES, INC.Inventors: Haoyun An, Xiaopeng Mo
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Patent number: 7732127Abstract: The present invention includes devices and methods for dynamically monitoring cell adhesion and cell spreading. Cells are added to a microelectronic cell sensor array operably connected to an impedance analyzer. The device also includes a coating including biological molecule or organic compound capable of interacting with the cell. Cell adhesion and cell mobility is determined by detecting changes in impedance and comparing impedance or cell index values between samples.Type: GrantFiled: September 27, 2005Date of Patent: June 8, 2010Assignee: ACEA Biosciences, Inc.Inventors: Xiaobo Wang, Yama Abassi, Josephine Atienza, Xiao Xu
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Patent number: 7622507Abstract: The present invention provides substituted di-, tri-, tetra- and penta-sulfide compounds and compositions, and methods of using the same for the treatment and/or prevention of a cell proliferative disorder. The present invention also provides methods for preparing trisulfide compounds and compositions.Type: GrantFiled: April 20, 2005Date of Patent: November 24, 2009Assignee: Acea Biosciences Inc.Inventors: Xiao Xu, Haoyun An, Xiaobo Wang
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Publication number: 20090205201Abstract: A device for detecting cells and/or molecules on an electrode surface is disclosed. The device detects cells and/or molecules through measurement of impedance changes resulting from the cells and/or molecules. A disclosed embodiment of the device includes a substrate having two opposing ends along a longitudinal axis. A plurality of electrode arrays are positioned on the substrate. Each electrode array includes at least two electrodes, and each electrode is separated from at least one adjacent electrode in the electrode array by an expanse of non-conductive material. The electrode has a width at its widest point of more than about 1.5 and less than about 10 times the width of the expanse of non-conductive material. The device also includes electrically conductive traces extending substantially longitudinally to one of the two opposing ends of the substrate without intersecting another trace. Each trace is in electrical communication with at least one of the electrode arrays.Type: ApplicationFiled: December 1, 2008Publication date: August 20, 2009Applicant: ACEA BIOSCIENCES, INC.Inventors: Xiao Xu, Xiaobo Wang
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Publication number: 20090192146Abstract: The present invention provides substituted di-, tri-, tetra- and penta-sulfide compounds and compositions, and methods of using the same for the treatment and/or prevention of a cell proliferative disorder. The present invention also provides methods for preparing trisulfide compounds and compositions.Type: ApplicationFiled: February 9, 2009Publication date: July 30, 2009Applicant: ACEA BIOSCIENCES, INC.Inventors: Xiao XU, Haoyun An, Xiaobo Wang