Abstract: The present invention relates to an optimized metabolic pathway design in P. pastoris. In particular, to a recombinant polycistronic expression construct for stable expression of multiple genes of interest in a yeast cell, preferably in P. pastoris.

Abstract: The invention relates to an isolated waterproof polymeric nanomembrane comprising pores of different geometric shapes and of a controlled size between 10 and 1000 nm, which is larger than the thickness of the membrane, and a method of producing the same comprising the process steps a. Providing a sacrifice layer on a surface of a solid support; b. Providing a polymerized layer of less than 1000 nm thickness on the surface of the sacrifice layer, by depositing a mixture of a polymer or a polymer precursor with a geometrically undefined pore template which is larger than the thickness of the polymerized layer, optionally followed by polymerization and/or crosslinking; c. Removing the pore template to obtain the polymerized layer with a controlled pore size; and d. Removing the sacrifice layer, thereby separating the solid support from the polymerized layer.