Abstract: Provided are methods of producing an acellular organ. The method includes the steps of, subjecting an organ derived from an animal to a static supercritical fluid (SCF) treatment followed by a dynamic SCF treatment. Optionally, the method of the present disclosure further includes a hypertonic and a hypotonic treatments prior to the static SCF treatment, and/or a neutralizing treatment after the dynamic SCF treatment. Also disclosed herein are acellular organs produced by the present method.
Abstract: Disclosed herein is a method of producing acellular cartilage grafts. The method includes steps of, subjecting a cartilage matrix derived from an animal to alkaline, disinfection and decelluarization treatments. The thus produced cartilage graft is devoid of any cellular matters, while maintaining the porosity and integrity of collagen fibers therein, thus is suitable as a xenograft for host cells to grown thereon. Also disclosed herein is a method for treating osteochondral disease of a subject, in which the present acellular cartilage graft is applied to a lesion site of the subject.
Abstract: Provided are methods of producing an acellular organ. The method includes the steps of, subjecting an organ derived from an animal to a static supercritical fluid (SCF) treatment followed by a dynamic SCF treatment. Optionally, the method of the present disclosure further includes a hypertonic and a hypotonic treatments prior to the static SCF treatment, and/or a neutralizing treatment after the dynamic SCF treatment. Also disclosed herein are acellular organs produced by the present method.
Abstract: Disclosed herein is a method of producing collagen particles. Each of the collagen particle is characterized in having a particle size of about 10-250 ?m, in which the integrity of collagen fibers therein is relatively intact.
Type:
Grant
Filed:
August 11, 2016
Date of Patent:
January 5, 2021
Assignee:
ACRO BIOMEDICAL COMPANY. LTD.
Inventors:
Jun-Jie Wang, Pei-Hua Tsai, Kai-Chi Ku, Dar-Jen Hsieh
Abstract: A method of producing an acellular cornea includes steps of subjecting a cornea of an animal to a decellularization process, and has not the step of treating the cornea with a protease, a chelating agent, a detergent, a glycerol, or a combination thereof. When a native cornea is processed by the method, the native structure and conformation of the native cornea are preserved while immunogenic matters are reduced to a level that the thus produced cornea may serve as a three-dimensional scaffold for host cells to grow thereon after transplantation.
Type:
Grant
Filed:
December 13, 2016
Date of Patent:
May 26, 2020
Assignee:
ACRO BIOMEDICAL COMPANY. LTD.
Inventors:
Shing-Jye Chen, Fan-Wei Tseng, Kai-Chi Ku, Dar-Jen Hsieh
Abstract: Disclosed herein is a method of producing collagen particles. Each of the collagen particle is characterized in having a particle size of about 10-250 ?m, in which the integrity of collagen fibers therein is relatively intact.
Type:
Application
Filed:
August 11, 2016
Publication date:
February 6, 2020
Applicant:
Acro Biomedical Company. Ltd.
Inventors:
Jun-Jie WANG, Pei-Hua TSAI, Kai-Chi KU, Dar-Jen HSIEH
Abstract: Disclosed herein is a method of producing acellular cartilage grafts. The method includes steps of, subjecting a cartilage matrix derived from an animal to alkaline, disinfection and decelluarization treatments. The thus produced cartilage graft is devoid of any cellular matters, while maintaining the porosity and integrity of collagen fibers therein, thus is suitable as a xenograft for host cells to grown thereon. Also disclosed herein is a method for treating osteochondral disease of a subject, in which the present acellular cartilage graft is applied to a lesion site of the subject.
Abstract: A method of producing an acellular cornea includes steps of subjecting a cornea of an animal to a decellularization process, and has not the step of treating the cornea with a protease, a chelating agent, a detergent, a glycerol, or a combination thereof. When a native cornea is processed by the method, the native structure and conformation of the native cornea are preserved while immunogenic matters are reduced to a level that the thus produced cornea may serve as a three-dimensional scaffold for host cells to grow thereon after transplantation.
Type:
Application
Filed:
December 13, 2016
Publication date:
October 18, 2018
Applicant:
Acro Biomedical Company. Ltd.
Inventors:
Shing-Jye CHEN, Fan-Wei TSENG, Kai-Chi KU, Dar-Jen HSIEH