Abstract: A novel method for fabricating glass micropipettes comprises assembling glass tubes side-by-side in a bundle, softening an axially central region of the bundle by heating, axially elongating the softened central region to create two opposing tapers, and then dividing the bundle in two at the axial midpoint of the central region to thereby create two micropipettes. The distal tip end of each micropipette at which it was separated from the other is then relatively advanced toward a hot flame to create a spherical enlargement at the tip end. This spherical enlargement facilitates subsequent gripping of this end when the micropipette is subsequently finished to produce desired orifice sizes for the barrels at the distal tip end. It is also desirable to perform a twisting step after the softening step and before the elongating step.