Abstract: The invention provides methods and kits for detecting conformationally altered proteins and prions in a sample. In one embodiment, the conformationally altered proteins and prions are associated with amyloidogenic diseases.
Type:
Application
Filed:
September 12, 2014
Publication date:
August 20, 2015
Applicant:
Adlyfe, Inc.
Inventors:
Cindy Orser, Anne Grosset, Eugene A. Davidson
Abstract: Disclosed are novel peptides that are useful, for example, for detecting target proteins having a ?-sheet secondary structure which may be associated with a disease, and for diagnosing and treating such a disease. Related methods and kits also are disclosed.
Type:
Application
Filed:
June 9, 2014
Publication date:
May 14, 2015
Applicant:
Adlyfe, Inc.
Inventors:
D. Roxanne Duan, Jonathan R. Moll, Alan Rudolph, Renee Wegrzyn
Abstract: Described are methods for the detection, in the eye of an individual, of protein aggregates or other misfolded proteins associated with disease using peptide or peptide mimic probes that preferentially associate with the protein aggregates or misfolded proteins, which can be accomplished non-invasively.
Abstract: Disclosed are agents and methods that may be used to diagnose and treat a variety of diseases associated with conformationally-altered proteins. The agents and methods may be used to identify and deliver drugs useful for treating diseases associated with conformationally-altered proteins.
Type:
Grant
Filed:
July 26, 2007
Date of Patent:
March 18, 2014
Assignee:
Adlyfe, Inc.
Inventors:
Cindy S. Orser, Alan Rudolph, Shankarrama Shivaprasad, Renee Wegrzyn
Abstract: The invention provides methods and kits for detecting conformationally altered proteins, such as prions or other proteins associated with disease states, in a sample. The methods comprise selectively capturing and separating complexes of peptide and conformationally altered protein from substances that interfere with detection of such complexes, and preferably amplification of the detection signal b addition of a second double-labeled peptide.
Abstract: A catalytic conformational sensor method for detecting abnormal proteins and proteinaceous particles. The method is based on the interaction of a peptide fragment or probe with an abnormal proteinaceous particle. The interaction catalyzes the transformation of the probe to a predominately beta sheet conformation and allows the probe to bind the abnormal proteinaceous particle. This in turn, catalyzes the propagation of a signal associated with the test sample-bound probe. As a result signals can be propagated even from samples containing very low concentrations of abnormal proteinaceous particles. The peptide probes can be designed to bind to a desired peptide sequence or can even be based on dendrimer structure to control further aggregate propagation.
Type:
Grant
Filed:
March 18, 2010
Date of Patent:
November 22, 2011
Assignee:
Adlyfe, Inc.
Inventors:
Cindy Orser, Anne Grosset, Eugene A. Davidson
Abstract: A catalytic conformational sensor method for detecting abnormal proteins and proteinaceous particles. The method is based on the interaction of a peptide fragment or probe with an abnormal proteinaceous particle. The interaction catalyzes transformation of the probe to a predominately beta sheet conformation and allows the probe to bind to the abnormal proteinaceous particle. This in turn, catalyzes propagation of a signal associated with the test sample-bound probe. As a result signals can be propagated even from samples containing very low concentrations of abnormal proteinaceous particles. The peptide probes can be designed to bind to a desired peptide sequence or can even be based on dendrimer structure to control further aggregate propagation.
Type:
Grant
Filed:
May 30, 2002
Date of Patent:
April 6, 2010
Assignee:
Adlyfe, Inc.
Inventors:
Cindy Orser, Anne Grosset, Eugene A. Davidson