Abstract: Apparatuses and methods for whole column imaging detection (WCID) capillary isoelectric focusing (CIEF). The apparatus includes a separation capillary having a separation inner diameter and a separation outer diameter; a base, wherein the separation capillary is anchored to the base; an inlet transfer capillary having an inlet inner diameter and an inlet outer diameter; and an outlet transfer capillary having an outlet inner diameter and an outlet outer diameter. The inlet transfer capillary, the separation capillary, and outlet transfer capillary are configured to be in fluidic communication with each other. The separation inner diameter exceeds the outlet inner diameter.