Abstract: In one embodiment, a system for biomedical object segmentation includes one or more processors; one or more memory modules communicatively coupled to the one or more processors, and machine readable instructions stored on the one or more memory modules. The machine readable instructions cause the system to perform the following when executed by the one or more processors: receive image data of one or more biological constructs; analyze the image data to generate processed image data via a data analytics module to recognize biomedical objects; and automatically annotate the processed image data to indicate a location of one or more biological objects within the one or more biological constructs.

Abstract: Modular pump assemblies according to the present disclosure include a plurality of mounting frames configured to be stackable with one another in a modular configuration, and an array of pumps mounted to each of the plurality mounting frames, the array of pumps comprising an array of inlet pumps configured to be fluidically coupled a plurality of fluid inlet paths of a well-plate manifold or an array of fluid outlet pumps configured to be fluidically coupled to a plurality of fluid outlet paths of the well-plate manifold, or any combination thereof.

Abstract: A storage assembly for storing a plurality of specimens includes a frame, a plurality of modular storage units for perfusion and/or incubation of one or more specimens removably coupled to the frame, a sample transfer apparatus configured to retrieve a specimen holder from a chosen modular storage unit of the plurality of modular storage units, and a control unit communicatively coupled to the sample transfer apparatus. The control unit is configured to cause the sample transfer apparatus to retrieve a specimen from a modular storage unit of the plurality of modular storage units and deliver the specimen to a delivery position.

Abstract: A bona fide adaptable in vitro microcirculation model is provided by integrating a 3-D printed network of endothelial-cell lined perfusion channels, formed via sacrificial casting in a gel matrix, with a native, adaptable microvasculature matured from native microvessels added to the gel matrix. Responsive vascular adaptation exhibited by the in vitro microcirculation is physiologically relevant. Methods for fabricating, devices, models and investigative platforms for pharmaceutical applications, vascular mechanism and microvessel-parenchyma interaction studies, and vascularizing strategies for tissue engineering applications are also disclosed.

Abstract: A bona fide adaptable in vitro microcirculation model is provided by integrating a 3-D printed network of endothelial-cell lined perfusion channels, formed via sacrificial casting in a gel matrix, with a native, adaptable microvasculature matured from native microvessels added to the gel matrix. Responsive vascular adaptation exhibited by the in vitro microcirculation is physiologically relevant. Methods for fabricating, devices, models and investigative platforms for pharmaceutical applications, vascular mechanism and microvessel-parenchyma interaction studies, and vascularizing strategies for tissue engineering applications are also disclosed.

Abstract: Methods and systems for designing a volumetric model of a construct at a user interface through use of a 3-D design, fabrication and assembly system comprising a modeling component, a robotic assembly workstation component, and a workflow configuration module to generate, through the workflow configuration module, a 3-D print bill of materials for a rendered volumetric model; generate a workflow configuration model based on the 3-D print bill of materials, the model including an automated robot control scheme of a series of assembly order instructions to direct a multi-axis robot of the robotic assembly workstation component to print and/or assemble a construct; transmit the workflow configuration model to the robotic assembly workstation component with a print and/or assembly command in accordance with the automated robot control scheme; and print and/or assemble the construct with the multi-axis robot in accordance with the print and/or assembly command.

Abstract: Methods and systems for 3D printing use a 3D printing device defined by a polar coordinate frame including an r-axis, a z-axis, and a rotational theta axis. The device includes a base, a rotatably attached printing stage is rotatably attached, a z-axis aligned pair of towers, an r-axis aligned rail slidably coupled to the towers, a print head slidably disposed on the rail, a printing tool coupling component (“master”) joined to the print head, and a rotatable tool carousel with bays housing printing tools, each including a printing tool body (“slave”). The slave may be coupled with and locked to or unlocked from the master to form a coupled tool assembly through a mechanical actuation assembly. With the coupled tool assembly, a printing tool is removable from a respective bay when the coupled tool assembly moves along the r-axis in a direction opposite from the rotatable tool carousel.

Abstract: An end effector calibration assembly includes an electronic controller, a first camera assembly communicatively coupled to the electronic controller, and a second camera assembly communicatively coupled to the electronic controller. A first image capture path of the first camera assembly intersects a second image capture path of the second camera assembly. The electronic controller receives image data from the first camera assembly, receives image data from the second camera assembly, and calibrates a position of the robot end effector based on the image data received from the first camera assembly and the second camera assembly.

Abstract: A well-plate assembly includes a well-plate defining an array of wells and a fluidic manifold assembly fitted to the array of wells and configured to direct a fluid into each well of the well plate.

Abstract: A bioassembly system having a tissue/object modeling software component fully and seamlessly integrated with a robotic bioassembly workstation component for the computer-assisted design, fabrication and assembly of biological and non-biological constructs. The robotic bioassembly workstation includes a six-axis robot providing the capability for oblique-angle printing, printing by non-sequential planar layering, and printing on print substrates having variable surface topographies, enabling fabrication of more complex bio-constructs including tissues, organs and vascular trees.

Abstract: A bioassembly system having a tissue/object modeling software component fully and seamlessly integrated with a robotic bioassembly workstation component for the computer-assisted design, fabrication and assembly of biological and non-biological constructs. The robotic bioassembly workstation includes a six-axis robot providing the capability for oblique-angle printing, printing by non-sequential planar layering, and printing on print substrates having variable surface topographies, enabling fabrication of more complex bio-constructs including tissues, organs and vascular trees.