Abstract: A method of extracting DNA from a biological sample includes the steps of freezing a biological sample; mechanically breaking down the sample to produce a processed sample; mixing the processed sample with a lysis solution; and cycling the mixture through a series of pressure fluctuations to produce a resulting lysate. The method may further include the steps of exposing the lysate to a material that binds to DNA present in the sample and collecting the DNA from the sample using an elution solution. An apparatus for practicing the method includes a first segment for mechanically breaking down the sample; a second segment for pressurizing a mixture of the processed sample and lysis solution to produce a lysate; and wherein the first segment is fluidly coupled to the second segment. The apparatus may also include a third segment in which the lysate is exposed to the binding material.
Type:
Grant
Filed:
June 12, 2015
Date of Patent:
June 12, 2018
Assignee:
AG-Defense Systems, Inc.
Inventors:
Rex Bryan Dyer, Beth Susan Turnbull-Dyer, Kenneth L. Owen, John G. Schierer
Abstract: The present invention is a portable device for detecting microorganisms in a food sample. The device includes a DNA extraction tube, a chip, and a portable control unit. The DNA extraction tube extracts DNA from the sample in an aqueous solution. The extraction tube includes successive chambers that are configured to mince, filter, and purify DNA in the form of an aqueous eluate. The chip includes reaction chambers disposed for a polymerase chain reaction to amplify a specific DNA fragment in the eluate for detection, and the portable control unit is configured to receive the chip and analyze the amplified DNA on the chip.
Type:
Application
Filed:
February 25, 2009
Publication date:
August 26, 2010
Applicant:
Ag-Defense Systems, Inc.
Inventors:
Rex Bryan Dyer, Beth Susan Turnbull-Dyer