Abstract: The invention relates to a method for amplification of a nucleic acid by a nucleic acid polymerase via two primers, each of which comprise a sequence segment which is bound to the target sequence and a sequence segment which does not bind to the target sequence, wherein the second cannot serve as a template for the polymerase, and two controller oligonucleotides, each of which is complementary to the primers and a segment of the sequence segment synthesized by them and serves to release the synthesized strand from the template. The controllers also comprise modified nucleotide building blocks such that they cannot serve as templates for the activity of the first template-dependent nucleic acid polymerase.

Abstract: The present invention relates to a method for the extension of an oligonucleotide primer with improved specificity using a specific oligonucleotide primer and a controller nucleotide, wherein the controller oligonucleotide enables sequence-specific strand opening of a double strand of extension product and template. The invention further relates to a respective kit for carrying out the method according to the invention.

Abstract: It is disclosed a method for the detection of an amplification of nucleic acids in which substantially use is made of the fact that a pre-defined nucleic acid chain (target sequence) can be multiplied/amplified in the presence of a target sequence-specific activator oligonucleotide. The target sequence-specific activator oligonucleotide causes the separation of re-synthesized complementary primer extension products by means of strand displacement, so that a new primer oligonucleotide can attach to the respective template strand. The thus formed complex of a primer oligonucleotide and a template strand can initiate a new primer extension reaction. The thus formed primer extension products in turn function as templates, so that an exponential amplification reaction results. Amplification is detected by a detection system.

Abstract: A method for amplification of nucleic acids in which substantially use is made of the fact that a pre-defined nucleic acid chain (target sequence) can be multiplied/amplified in the presence of a target sequence-specific activator oligonucleotide. The target sequence-specific activator oligonucleotide causes the separation of re-synthesized complementary primer extension products by strand displacement, so that a new primer oligonucleotide can attach to the respective template strand. The thus formed complex of a primer oligonucleotide and a template strand can initiate a new primer extension reaction. The thus formed primer extension products in turn function as templates, so that an exponential amplification reaction results.

Abstract: The invention relates to a novel method for the enzymatic marking of nucleic acid chains (target sequences) by means of nucleotide conjugates. Said nucleotide conjugates are able, under reaction conditions, to specifically bind to the target sequence and integrate into the complementary growing strand by means of a polymerase. The nucleic acid chains marked by such conjugates can be bound to the solid phase. The marking can be carried out parallel to the enzymatic amplification of target sequences.