Abstract: A process of concentration by precipitation of PrPsc for detecting or diagnosing PrPsc, wherein a tissue or biological fluid stemming from or obtained from an animal or human organism is brought together with an antibiotic from the family of aminoglycosides, preferably streptomycin or one of its derivatives. The use of such an antibiotic for eliminating PrPsc from a tissue or biological fluid and a kit for diagnosing pathologies associated with the presence of PrPsc is also disclosed.
Type:
Grant
Filed:
June 13, 2005
Date of Patent:
April 13, 2010
Assignees:
Agence Francaise de Securite Sanitaire des Aliments-AFSSA, Centre National de la Recherche Scientifique-CNRS, Universite Claude Bernard Lyon, Biomerieux
Abstract: A process for detecting the forms of the prion pathogens responsible for subacute, transmissible, spongiform encephalopathies, including a macrocyclic adjuvant ligand (AML), free or bound to a support, that is added to a biological sample capable of containing PrPsc, the resulting suspension then being reacted with an anti-PrPsc antibody, and the presence of PrP is then detected.
Type:
Application
Filed:
February 1, 2007
Publication date:
July 5, 2007
Applicants:
Agence Francaise de Securite Sanitaire Des Aliments-AFSSA, Centre National de la Recherche Scientifique-CNRS, Universite Claude Bernard Lyon
Inventors:
Aly Moussa, Anthony Coleman, Patrick Shahgaldian, Eric Da Silva, Ambroise Martin, Adina Lazar, Edwige Leclere, Marilyne Dupin, Herve Perron
Abstract: A process for detecting the forms of the prion pathogens responsible for subacute, transmissible, spongiform encephalopathies, including a macrocyclic adjuvant ligand (AML), free or bound to a support, that is added to a biological sample capable of containing PrPsc, the resulting suspension then being reacted with an anti-PrPsc antibody, and the presence of PrP is then detected.
Type:
Grant
Filed:
June 9, 2005
Date of Patent:
May 15, 2007
Assignees:
Agence Francaise de Securite Sanitaire des Aliments-AFSSA, Centre National de la Recherche Scientifique-CNRS, Universite Claude Bernard Lyon and Biomerieux
Inventors:
Aly Moussa, Anthony William Coleman, Patrick Shahgaldian, Eric Da Silva, Ambroise Martin, Adina Nicoleta Lazar, Edwige Leclere, Marilyne Dupin, Herve Perron
Abstract: A process for detecting the forms of the prion pathogens responsible for subacute, transmissible, spongiform encephalopathies, including a macrocyclic adjuvant ligand (AML), free or bound to a support, that is added to a biological sample capable of containing PrPsc, the resulting suspension then being reacted with an anti-PrPsc antibody, and the presence of PrP is then detected.
Type:
Application
Filed:
June 9, 2005
Publication date:
January 26, 2006
Applicants:
Agence Francaise de Securite Sanitaire des Aliments-AFSSA, an organization of France, Centre National de la Recherche Scientifique-CNRS, an organization of France, Universite Claude Bernard Lyon, an organization of France, Biomerieux, an organization of France
Inventors:
Aly Moussa, Anthony Coleman, Patrick Shahgaldian, Eric Silva, Ambroise Martin, Adina Lazar, Edwige Leclere, Marilyne Dupin, Herve Perron
Abstract: A process of concentration by precipitation of PrPsc for detecting or diagnosing PrPsc, wherein a tissue or biological fluid stemming from or obtained from an animal or human organism is brought together with an antibiotic from the family of aminoglycosides, preferably streptomycin or one of its derivatives. The use of such an antibiotic for eliminating PrPsc from a tissue or biological fluid and a kit for diagnosing pathologies associated with the presence of PrPsc is also disclosed.
Type:
Application
Filed:
June 13, 2005
Publication date:
January 19, 2006
Applicants:
Agence Francaise de Securite Sanitaire des Aliments-AFSSA, an organization of France, Centre National de la Recherche Scientifique-CNRS, an organization of France, Universite Claude Bernard Lyon, an organization of France, Biomerieux, an organization of France
Inventors:
Aly Moussa, Anthony Coleman, Anna Bencsik-Reynier, Patrick Shahgaldian, Herve Perron, Ambroise Martin