Abstract: The present invention is directed to a method of isolating a target species (e.g., target nucleic acid species) from a mixture. In the methods of the present invention, the mixture is combined with solid phase carriers having a surface comprising multiple functional groups one of which reversibly and selectively binds the target species. In a particular embodiment, the mixture is combined with solid phase carriers having a first functional group which reversibly binds nucleic acids and a second functional group which selectively and reversibly binds the target nucleic acid species, thereby producing a first combination. The first combination is maintained under conditions appropriate for binding of the nucleic acids to the first functional group and binding of the target nucleic acid species to the second functional group. The solid phase carriers are separated from the first combination, and combined with an agent (e.g., buffer) that selectively removes (e.g.

Abstract: The present invention is directed to a method of isolating a target species (e.g., target nucleic acid species) from a mixture. In the methods of the present invention, the mixture is combined with solid phase carriers having a surface comprising multiple functional groups one of which reversibly and selectively binds the target species. In a particular embodiment, the mixture is combined with solid phase carriers having a first functional group which reversibly binds nucleic acids and a second functional group which selectively and reversibly binds the target nucleic acid species, thereby producing a first combination. The first combination is maintained under conditions appropriate for binding of the nucleic acids to the first functional group and binding of the target nucleic acid species to the second functional group. The solid phase carriers are separated from the first combination, and combined with an agent (e.g., buffer) that selectively removes (e.g.

Abstract: Methods for producing a paired tag from a nucleic acid sequence are provided in which the paired tag comprises the 5? end tag and 3? end tag of the nucleic acid sequence. In one embodiment, the nucleic acid sequence comprises two restriction endonuclease recognition sites specific for a restriction endonuclease that cleaves the nucleic acid sequence distally to the restriction endonuclease recognition sites. In another embodiment, the nucleic acid sequence further comprises restriction endonuclease recognition sites specific for a rare cutting restriction endonuclease. Methods of using paired tags are also provided. In one embodiment, paired tags are used to characterize a nucleic acid sequence. In a particular embodiment, the nucleic acid sequence is a genome. In one embodiment, the characterization of a nucleic acid sequence is karyotyping. Alternatively, in another embodiment, the characterization of a nucleic acid sequence is mapping of the sequence.

Abstract: A method and device for introducing a sample, such as a nucleic acid sample, into an electrophoretic apparatus is provided in which the sample is introduced into the electrophoretic apparatus in the presence of a magnetic field. In a particular embodiment, the electrophoretic apparatus can include a nucleic acid sequencer. The sample can be introduced into the electrophoretic apparatus electrokinetically. The magnetic field attracts one or more magnetic microparticles that can be suspended or otherwise provided in the sample. The nucleic acid sample can be bound to one or more magnetic microparticles. In particular embodiments, the microparticles can be used to purify dye terminator sequencing reactions. The magnetic field can be formed by a rare earth magnet, such as neodymium, or by an electromagnet, or other suitable means. In particular embodiments, the sample can be injected into a nucleic acid sequencer by capillary electrophoresis and sequenced by the sequencer.

Abstract: The present invention relates to isolated nucleic acids that encode polypeptides that interact with T4SS (referred to herein as “T4SS interactor nucleic acids” and “T4SS interactor polypeptides”) and complements, orthologs, portions and variants thereof. The present invention also relates to isolated T4SS interactor polypeptides, orthologs and portions thereof, and antibodies or antigen binding fragments thereof that specifically bind a T4SS interactor polypeptide. The present invention also relates to constructs and host cells comprising the nucleic acid molecules described herein. In addition, the present invention relates to uses of the nucleic acid and polypeptide molecules provided herein.

Abstract: Described herein is a method in which genomic nucleic acid of a cell can be separated from nucleic acid having a molecular weight that is lower than the molecular weight of the genomic nucleic acid (e.g., plasmid DNA) of the cell directly from a cell growth culture. Also described herein, a method in which genomic nucleic acid can be separated from nucleic acid having a molecular weight that is lower than the molecular weight of the genomic nucleic acid in a cell lysate without the need to prepare a cleared lysate.

Abstract: The present invention provides methods for determining the sequence of nucleic acids encoding interacting polypeptide sequences. Two hybrid assays are carried out to select host cells containing nucleotide sequences encoding interacting proteins. The identity of the nucleotide sequences is determined by isolating the nucleotide sequences from the selected host cells and carrying out sequencing reactions on the nucleotide sequences.