Patents Assigned to AgrEvo Canada, Inc.
  • Patent number: 6316694
    Abstract: The invention relates to transformed, embryogenic microspores and progeny thereof characterized by being transformed by Agrobacterium tumefaciens, capable of leading to non-chimeric transformed haploid or doubled haploid embryos that develop into fertile homozygous plants within one generation and containing stably integrated into their genome a foreign DNA, said DNA being characterized in that it comprises at least one gene of interest and at least base pairs within the right border sequence of Agrobacterium T-DNA. The invention furthermore relates to a method for the incorporation of foreign DNA into chromosomes of microspores comprising the following steps: a) infecting of embryogenic microspores with Agrobacteria, which contain plasmid carrying a gene of interest under regulatory control of initiation and termination regions bordered by at least one T-DNA border, b) Washing out and killing the Agrobacteria after co-cultivation.
    Type: Grant
    Filed: November 14, 1997
    Date of Patent: November 13, 2001
    Assignee: AgrEvo Canada, Inc.
    Inventors: Mathias Dormann, Hung-Mei Wang, Michael Oelck